PML基因诱导角质形成细胞凋亡及其Fas,Fasl和Bcl-2表达  

Study on PML Gene Induce Apoptosis of Keratinocytes and Expression of Fas,Fasl and Bcl-2 in the Keratinocytes

作　　者：王琼玉[1] 马慧群[1] 王世捷[2] 马云云[1] 邹兴伟[1] 刘平[1] 彭振辉[1] 

机构地区：[1]西安交通大学医学院第二附属医院皮肤科,陕西西安710004 [2]西安交通大学环境与疾病基因教育部重点实验室/环境与地方病研究室,陕西西安710061

出　　处：《中国皮肤性病学杂志》2013年第6期550-552,596,共4页The Chinese Journal of Dermatovenereology

基　　金：陕西省科技攻关项目(2008K12-02-5);国家自然科学基金(81171503)

摘　　要：目的探讨PML基因在银屑病发病机制中的作用。方法通过流式细胞仪及Annexin V法检测经PML基因转染的角质形成细胞凋亡;采用免疫组织化学、基因探针杂交测定经PML基因作用后的角质形成细胞Fas,Fasl和Bcl-2表达。结果 PML基因上调角质形成细胞Fas及Fasl表达(P<0.01),抑制Bcl-2基因表达,诱导角质形成细胞凋亡。结论 PML基因可能通过上调角质形成细胞Fas抗原及Fasl表达,抑制Bcl-2基因表达,进而诱导角质形成细胞凋亡而参与银屑病的发病。Objective To investigate the role of PML gene in the pathogenesis of psoriasis. Methods Study the apoptosis of keratinocyte transfected by PML endemic ergotism with flow cytometric analysis quantification and Annexin V method ; The expression of Fas, Fasl and Bcl-2 on keratinocyte transfected by PML gene were detected by SP immunohistochemistry and gene probe hibradization. Results PML gene upregulates the expression of Fas antigen and Fasl on the keratinocytes （ P 〈 O. 01 ） , downregulates the expression of Bcl-2 gene,induce apoptosis of keratinocytes. Conclusion PML gene upregulates the expression of Fas antigen and Fasl on the keratinocytes, downregulates the expression of Bcl-2 gene,induce apoptosis of keratinocytes , which might be involved in the pathogenesis of psoriasis.

关 键 词：PML基因 角质形成细胞 凋亡 FAS FASL Bcl-2 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]