不同剂量可卡因对昆明小鼠成瘾行为学的影响  

Various effect of dose-dependent cocaine on the addicted behavior of KM mice

作　　者：陈振中[1,2] 顾晶晶[1,2] 李娟[1,2] 张磊[3] 崔梦卿[4] 李良平[5] 张璐[1,2] 

机构地区：[1]南方医科大学病理生理学教研室 [2]广东省功能蛋白质组学重点实验室 [3]南方医科大学组织学与胚胎学教研室,广州510515 [4]南方医科大学南方医院门诊部,广州510515 [5]广州市红十字会医院神经外科,广州510220

出　　处：《中国临床解剖学杂志》2013年第3期325-327,共3页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(81071120);教育部新世纪优秀人才支持计划(NCET-09-0088);教育部科学技术研究重点项目(21132);粤港关键领域重点突破项目(2011A011304001);广东省产学研合作重大科技专项(2011A090100025);广州市科技计划项目(7411832133935);广东省自然科学基金(10151022001000003)

摘　　要：目的研究昆明小鼠对不同剂量可卡因的敏感度，为昆明小鼠在神经科学领域的应用，制别是可卡因成瘾的研究提供参考。方法同龄（8～12周）的雄性昆明小鼠，分别腹腔注射10mg／kg、2qmg／kg的可卡因，条件位置偏爱实验（Conditioned place preference）和行为敏化实验（Behavior sensitization检NA,鼠行为学变化，观察昆明小鼠在不同剂量可卡因刺激下的行为学变化，以此来确定昆明小鼠对不每剂量可卡因的敏感度。结果在条件位置偏爱实验中，可卡因20mg／kg剂量组（N=11）与可卡因10mg／kg剂量组（N=12）相比，第9天与第2天在白箱中的时间差score（post-pre）有统计学差异（P〈0．05）；而在行为敏化实验中，可卡因20mg／kg组（N=8）与可卡因1Omg／kg组（N=12）相比，自主活动次数明显增加，蓓统计学差异（P〈0．05）；在第16天给予一针与之前同剂量的可卡因，可卡因20mg／kg组与可卡因1Omg／kg组相比，自主活动次数有统计学差异（P〈0．05）。结论昆明小鼠在20mg/kg可卡因的刺激下，能产生要为明显的条件位置偏爱以及更加强烈的行为敏化反应，是较为理想的可卡因成瘾小鼠模型。Objective To investioate the sensitivity ofKunmin9 mice to different doses of cocaine, and provide reference for the cocaine related research of Kunmin9 mice. Methods Kunmino mice, male, aoed 8-12 weeks, were used. They were randomly divided into two groups. One 9roup was injected with 10 mg/kg cocaine, while the other 20 mg/kg cocaine. Conditioned place preference and Behavior sensitization were used to measure the behavioral differences before and after cocaine treatment. Results In the conditioned place preference analysis, the time difference in the white box on day 9 and day 2 （post-pre） was statistically siGnificant （P〈0.05） between the two groups. In the Behavior sensitization test, the ambulatory accounts between the two 9roups were found to be statistically siGnificant（P〈0.001 ）durin9 the time treated with cocaine. And then they were put back to the colony room for 1 week before they received a challenoino dose of cocaine. On the challenoin9 day, the ambulatory accounts were found to be statistically siGnificant between them （P=0.001）. Conclusion Kunmin9 mice can produce more obvious conditioned place preference and more intense behavioral sensitization reaction when treated with 20 mg/kg cocaine.

关 键 词：昆明小鼠 剂量 条件位置偏爱 行为敏化 

分 类 号：R338[医药卫生—人体生理学]