外周血滤泡辅助性T细胞百分率的变化与系统性红斑狼疮疾病相关性的初步研究  

A preliminary study of the correlation between the percentage of follicular helper T cells in peripheralblood and systemic lupus erythematosus disease

作　　者：尤海燕[1] 马红[2] 焦志军[1] 裘影影[3] 吴玲[3] 王蓓[1] 邹晓月[1] 

机构地区：[1]江苏大学附属医院检验科,镇江212001 [2]江苏大学附属医院皮肤科 [3]江苏大学附属医院风湿科

出　　处：《中华风湿病学杂志》2013年第6期396-399,共4页Chinese Journal of Rheumatology

基　　金：江苏大学临床医学科技发展基金（JLY2010119）

摘　　要：目的探讨滤泡辅助性T细胞（Tfh）在系统性红斑狼疮（SLE）患者外周血中百分率的变化及意义。方法采用流式细胞术检测60例SLE患者（其中活动期患者30例，稳定期患者30例）和30名健康体检者（对照组）外周血中cD4＋CXCR5＋1COS＋硼，细胞的百分率及Tfh细胞的活化标志物CD69的表达百分率。比较SLE患者外周血硼，细胞百分率与SLE疾病活动性指数（SLEDAI）、抗核抗体滴度及补体C3、C4水平之间的相关性。统计学处理采用单因素方差分析和Pearson相关分析。结果SLE活动组外周血孙细胞百分率[（0．80±0．17）％]显著高于SLE稳定组[（O．63±0．13）％]及健康对照组[（0．57±0．08）％]（P均〈O．01），SLE稳定组亦高于健康对照组（P〈0．05）。SLE活动组外周血Tfh细胞cD69的表达百分率[（7．3±1．6）％]显著高于SLE稳定组[（5．9±1．3）％]及健康对照组[（5．2±0．9）％]（P均〈0．01），SLE稳定组与健康对照组差异无统计学意义（P〉0．05）。SLE患者外周血Tfh细胞百分率与SLEDAI呈正相关（r=0．680，P〈0．01），与补体C3水平呈负相关（r=-0.416，P〈0．05），与ANA滴度及补体C4水平无明显相关性（r=-0．042，r=-0．204，P均〉0．05）。结论SLE患者外周血中Tfh，细胞百分率增多及活性增强可能与SLE的发生及发展有关。Objective To investigate the change and significance of T follicular helper cells（Tfh） in the peripheral blood of patients with systemic lupus erythematosus （SLE）. Methods The percentage of CD4＋ CXCR5＋ICOS＋ Tfh cells and the expression of activation marker CD69 on the Tfh cells of peripheral blood from 60 SLE patients （30 in active and 30 in inactive） and 30 healthy subjects （control group） were determined by flow cytometry. The correlations between the percentage of Tfh cells of SLE patients and SLE disease activity index （SLEDAI）, anti-nuclear antibody （ANA） titers and the levels of complement 3（C3）, complement 4 （C4） were analyzed. ANOVA and Pearson＇s correlation analysis were used for statistical analysis. Results The percentage of the Tfh cells in the peripheral blood of active SLE patients was higher than inactive patients and healthy controls [（0.80±0.17）% vs （0.63i±0.13）% vs （0.57±0.08）%; P〈0.01 ], there was also statistical significance between inactive patients and healthy controls （P〈0.05）. The expression of CD69 on the Tfh cells in the peripheral blood of active SLE patients was higher than that in inactive and healthy controls [（7.3 ±1.6）% vs （5.9±1.3）% vs （5.2±0.9）%; P〈0.01]. There was no statistical significant difference between inactive patients and healthy controls （P〉0.05）. The percentage of Ttla cells of SLE patients was significantly related with SLEDAI （r=0.680, P〈0.01） and C3 levels （r=-0.416, P〈0.05）, butthere was no correlation between that and the ANA titer, C4 levels （r=-0.042, -0.204, P〉0.05）. Conclusion Increased percentage and activity of the Tfh ceils in the peripheral blood of patients might contribute to the pathogenesis and development of SLE.

关 键 词：T淋巴细胞 辅助诱导 红斑狼疮 系统性 流式细胞术 

分 类 号：R593.241[医药卫生—内科学]