异体骨预制骨皮瓣的实验研究  

An experimental study for prefabrication of skin flap with frozen allogeneic bone

作　　者：栗威[1] 韩清銮[1] 张玉[1] 陈磊[1] 荣存敏[1] 陈菁华[1] 

机构地区：[1]济宁医学院附属医院手外科,山东省济宁市272029

出　　处：《中华显微外科杂志》2013年第3期257-260,共4页Chinese Journal of Microsurgery

基　　金：山东省自然科学基金（ZR2009CL010）;山东省济宁市科技发展计划项目（济科字2009-56号）

摘　　要：目的探讨异体骨异位埋置预制骨皮瓣的可行性。方法从2010年11月至2011年7月，用广西巴马小型猪为实验动物，先制作深低温冷冻异体骨备用；异体骨经复温后置于旋髂浅动脉供养的皮瓣组织内，按植入部位不同分3组：皮下组（A组）、深筋膜下组（B组）、肌内组（c组），术后培养观察。指标：异体骨ECT显像（分别于术后第4周、第8周、第12周），血管造影检查、异体骨病理学检查（术后第12周）。结果3组预制骨皮瓣术后ECT扫描显示：第8周和第12周异体骨植入部位放射性较4周时增高，差异有统计学意义（P〈0．05）；血管造影显示在皮下组织、筋膜下组织、肌肉组织内的异体骨均有血管化，并与相应区域旋髂浅动脉建立血管联系；病理检查可见异体骨在筋膜下组织和肌肉组织内血管化作用和诱导新骨形成较明显。结论可以用异体骨异位埋置预制骨皮瓣。Objective To research the feasibility of the prefabrication of skin flap with frozen allogeneic bone. Methods From November 2010 to July 2011, fifteen miniature pigs were randomly divided into three groups （ n = 5 ） according to different implanted positions. Rewarining, deep-frozen allogenic bone was implanted in subcutaneous tissue, subfascial compartment and muscle respectively. Emission Computed Tomography （ECT） was used in the 4th week, the 8th week and the 12th week postoperatively. Angiography and pathological examination was used in the 12th week. Results The difference of the result of ECT was statistically significant betweent the 4th week and the 8th week （ P 〈 0. 05 ）, and there was not statistically significant difference betweent the 12th week and the 8th week （P 〉 0. 05 ）. Angiography showed that all allogeneic bone was vascularized in each group. Pathological examination showed that the vascularization and the inducing osteogenesis of the allogeneie bone which was implanted in the subfaseial compartment or muscle was better than that in the subcutaneous tissue. Conclusion The deep-frozen allogenie bone can be used for the prefabrication of the bone-skin flap.

关 键 词：异体骨 预制 骨皮瓣 猪 

分 类 号：R65[医药卫生—外科学]