不结球白菜花芽分化分级及叶绿体色素含量的变化  

Flower Bud Differentiation Classification and Changes of Chloroplast Pigment Contents in Non-Heading Chinese Cabbage

作　　者：王真真[1] 侯瑞贤[1] 李晓峰[1] 朱红芳[1] 朱玉英[1] 

机构地区：[1]上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海201403

出　　处：《植物生理学报》2013年第6期568-572,共5页Plant Physiology Journal

基　　金：上海绿叶蔬菜产业技术体系项目;上海市科技攻关项目(09391911400)

摘　　要：以不结球白菜为试验材料,通过制作石蜡切片和显微镜观察,将花芽分化过程划分为6级:生长锥伸展期、花原基分化初期、花原基伸长期、花萼原基分化期、雄蕊原基分化期、雌蕊和花瓣原基分化期。早抽薹材料09-1-328c叶片中叶绿素和类胡萝卜变化趋势一致,呈现缓慢升高,抽薹期降低的趋势;晚抽薹材料08-1P-89和中等耐抽薹材料08-1P-76色素变化趋势一致,在花芽分化时降低,完成分化后升高,抽薹期又下降。叶绿素a/b在早抽薹材料中呈现降低趋势,在晚抽薹材料中保持较稳定水平,叶绿素(a+b)/类胡萝卜素在花芽分化时比值降低,现蕾期又升高。Non-heading Chinese cabbage （Brassica campestris ssp. chinensis Makino） was used as the test material. By means of paraffin seations and optical microscope, the flower bud differentiation process was divided into six classes： growth cone extension stage, the early stage of floral primordia differentiation, floral primordia elongation stage, sepal primordium differentiation stage, stamen primordium differentiation stage, pistil and petal primordium differentiation stage. The contents of chlorophyll and carotenoid in early bolting material 09-1-328c leaves showed the same trend, increased slowly, fell at bolting stage. As for late bolting material 08-1P-89 and secondary resistance to bolting marterial 08-1P-76, the trends were similar, chlorophyll and carotenoid contents fell at flower bud differentiation, increased when the differentiation completed, but fell at bolting stage. Chlorophyll a/b ratio presented a decreased tendency in early bolting material, and a maintained stable level in late bolting materials. Chlorophyll （a＋b）/carotenoid ratio fell in the process of flower bud differentiation, increased at flower bud emergence stage.

关 键 词：不结球白菜 花芽分化 抽薹 叶绿素 类胡萝卜素 

分 类 号：S634[农业科学—蔬菜学]