人脐血对兔骨折愈合期间全血微量元素含量的影响  

EFFECT OF HUMAN UMBILICAL CORD BLOOD ON CONTENT OF TRACE ELEMENTS IN WHOLE BLOOD DURING FRACTURE HEALING IN RABBITS

作　　者：李永乐[1] 何强[2] 范先东[2] 刘宝平[2] 李铁军[2] 孙义忠[2] 

机构地区：[1]河北北方学院基础医学院,河北张家口075000 [2]解放军第251医院骨科

出　　处：《中国修复重建外科杂志》2013年第6期673-679,共7页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的研究人脐血对兔骨折愈合过程中全血微量元素含量的影响,探讨其促进骨折愈合的机制。方法取4～5月龄新西兰大白兔63只,体重2.0～2.5 kg,制备右后肢胫骨横断骨折模型,根据处理方法不同,随机分为脐血组(A组)、生理盐水组(B组)和模型对照组(C组),每组21只。术后3、8 d,A组于骨折断端注射3 mL人脐血,B组注射等量生理盐水;C组不作任何处理。术后1、2、3、4、5、6周进行X线片、组织学观察,检测全血中锌、铜、镁、铁、钙、磷元素含量。结果 X线片示A组骨折愈合速度明显快于B、C组;术后2～6周A组X线评分显著高于B、C组,比较差异有统计学意义(P<0.05)。组织学观察示,A组新生骨小梁及类骨质明显多于B、C组;术后2～5周A组组织学评分均显著高于B、C组(P<0.05);术后6周,A组组织学评分高于B组(P<0.05),但与C组比较差异无统计学意义(P>0.05)。术后3组微量元素含量变化趋势基本一致:铜含量先降低后逐渐回升;锌、镁、铁含量均在不同时间点降低,但基本稳定;钙含量先升高后降低;磷含量先降低后升高。A组铜、锌、镁、铁、钙、磷含量在不同时间点显著高于B、C组,比较差异有统计学意义(P<0.05);B、C组间比较差异均无统计学意义(P>0.05)。结论骨折断端注射人脐血能明显减缓全血中微量元素的丢失,保证了骨折愈合过程中必需微量元素含量,这可能是脐血促进骨折愈合机制之一。Objective To study the effect of the human umbilical cord blood on the content of trace elements in whole blood during fracture healing in rabbits and to explore the mechanism of promoting fracture healing.Methods The right tibial fracture model was made in 63 white New Zealand rabbits（aged,4-5 months;weighing,2.0-2.5 kg）.The fracture site was treated with 3 mL human umbilical cord blood（group A,n=21） and 3 mL normal saline（group B,n=21） at 3 and 8 days after operation,and was not treated as a control（group C,n=21）.At 1,2,3,4,5,and 6 weeks after operation,the X-ray and histological observations were done;the contents of zinc,copper,magnesium,ferrum,calcium,and phosphorus were detected.Results X-ray observation showed that the fracture healing speed of group A was significantly faster than that of groups B and C;the fracture healing X-ray score of group A was significantly higher than that of groups B and C at 2-6 weeks（P？ 0.05）.The histological observation indicated that new trabeculae and osteoid of group A were significantly more than those of groups B and C;at 2-5 weeks,the histological score of group A was significantly higher than that of groups B and C（P？ 0.05）;at 6 weeks,the score of group A was significantly higher than that of group B（P？ 0.05）,but no significant difference was found between groups A and C（P 0.05）.Changes trend of the trace elements in 3 groups after operation was basically consistent.The content of copper first decreased and then gradually increased;the contents of ferrum,zinc,and magnesium at different time points decreased,but were basically stable;the content of calcium first increased and then decreased;the content of phosphorus first decreased and then increased.The contents of copper,zinc,magnesium,ferrum,calcium,and phosphorus in group A were significantly higher than those in groups B and C at different time points（P 0.05）,but there was no significant difference between groups B and C（P 0.05）.Conclusion Injection of the human umbilica

关 键 词：脐血 胫骨骨折 微量元素 全血 兔 

分 类 号：R683[医药卫生—骨科学]