大鼠脊髓损伤减压后神经功能恢复与Fas表达的相关性研究  

RELATIONSHIP BETWEEN Fas EXPRESSION AND RECOVERY OF NEUROLOGICAL FUNCTION AFTER SURGICAL DECOMPRESSION IN SPINAL CORD INJURY RAT MODEL

作　　者：李全辉[1,2] 顾文浩[2] 谢加兵[2] 徐祝军[2] 

机构地区：[1]颍上县人民医院骨二科安徽颍上,236200 [2]皖南医学院附属弋矶山医院创伤骨科

出　　处：《中国修复重建外科杂志》2013年第6期708-714,共7页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：安徽省卫生厅2009年度医学研究课题资助项目(09C33)~~

摘　　要：目的通过环扎法制备大鼠急性脊髓损伤(spinal cord injury,SCI)模型,研究不同时间点减压后细胞凋亡基因Fas的表达规律及与神经功能恢复的关系。方法 13周龄雄性SD大鼠100只,体重255～376 g,随机分为4组,每组25只。A组为对照组,仅行椎板切除;B、C、D组均采用环扎法建立急性SCI模型,B、C组分别于术后8、72 h行脊髓减压,D组术后不行脊髓减压。于术后1、3、7、14、21 d采用BBB评分及斜板实验评分评价神经功能恢复情况;处死动物取材行HE染色、免疫组织化学染色观察Fas表达,TUNEL标记观察细胞凋亡水平。结果 A组术后各时间点BBB评分和斜板实验评分均明显优于B、C、D组,3、7、14、21 d时B组优于C、D组,C组优于D组,差异均有统计学意义(P<0.05)。A组各时间点脊髓组织结构及形态清楚,未见出血、水肿和变性坏死的神经元。术后1 d,B、C、D组脊髓组织明显出血、水肿,可见神经元死亡,D组较显著;3、7 d,B组细胞变性较C、D组轻;14、21 d,B组损伤区可见较少胶质细胞,有纤维母细胞增生和修复现象,C、D组脊髓内有部分坏死、囊腔及空洞形成。A组术后各时间点均少见Fas和TUNEL阳性细胞;B、C、D组术后各时间点均见Fas和TUNEL阳性细胞,3 d达峰值,7 d表达减弱,21 d仍有表达;其中D组阳性细胞数最多,B组少于C、D组,差异均有统计学意义(P<0.05)。结论 SCI后早期减压有利于大鼠神经功能的恢复,Fas信号通道可能在诱导SCI后神经细胞凋亡的过程中发挥重要作用。Objective To investigate the relationship between the expression of apoptosis-related gene Fas and recovery of neurological function after surgical decompression at different time points in acute spinal cord injury（SCI） rat model by cerclage.Methods A total of 100 13-week-old male Sprague Dawley rats（weighing,255-376 g） were randomly divided into 4 groups（n=25）.The rats only received laminectomy in group A as control;the rats were made the acute SCI models by cerclage in groups B,C,and D.The spinal cord decompression was performed in group B at 8 hours and in group C at 72 hours,no spinal cord decompression in group D.At 1,3,7,14,and 21 days,Basso-Beattie-Bresnahan（BBB） score and inclined plane test were used to evaluate the recovery of neurological function;the neuronal apoptosis level of spinal cord was examined by TUNEL staining;HE staining and immunohistochemical staining were applied to analyze the expressions of Fas.Results The BBB score and inclined plane test score in group A were significantly better than those in groups B,C,and D at different time points（P 0.05）;group B was significantly better than groups C and D,and group C than group D at 3,7,14,and 21 days（P 0.05）.In group A,no bleeding,edema,or necrosis was found.The edema,hemorrhage,and neuron death were observed in spinal cord tissue of groups B,C,and D at 1 day after operation,especially in group D.The degree of cell degeneration in group B was lighter than that in groups C and D at 3 and 7 days after operation;few glial cells and fibroblast proliferation were found at damaged zone in group B at 14 and 21 days,but necrosis and cystic cavity in groups C and D.Fas and TUNEL expression was little in group A at different time points.Fas and TUNEL were expressed in groups B,C,and D;the expressions of Fas and TUNEL reached the maximum at 3 days,and then gradually decreased at 7 and 21 days.The number of positive cells was highest in group D,and the number of positive cells in group B was significantly less than that in groups C

关 键 词：脊髓损伤 神经减压 FAS 细胞凋亡 大鼠 

分 类 号：R651.2[医药卫生—外科学]