脑损伤后脑脊液中基质金属蛋白酶-9的临床意义研究  

Clinical significance of matrix metalloproteinases-9 content in cerebrospinal fluid of brain injury

作　　者：于辉天[1] 叶华卫[1] 麦荣康[1] 江先福[1] 宋彧[1] 赖菊芳 史小军[2] 

机构地区：[1]深圳市宝安区人民医院神经外科,510810 [2]清华大学深圳研究生院生命学部,深圳518055

出　　处：《中华神经医学杂志》2013年第6期636-638,共3页Chinese Journal of Neuromedicine

摘　　要：目的探讨脑损伤后脑脊液中基质金属蛋白酶．9（MMP-9）含量变化及其临床意义。方法临床收集深圳市宝安区人民医院神经外科自2010年1月至2012年6月收治的69例不同类型脑损伤患者脑脊液标本为实验组，另外选取35例健康者脑脊液标本为对照组。采用ELISA法检测实验组24h、72h、1周及2周时脑脊液中MMP．9的含量变化，并与对照组进行比较分析。结果测试发现实验组MMP．9在脑脊液中的活性明显升高，可能与脑损伤程度、脑水肿发生以及脑损伤后疾病的转归有密切关系。实验组所有脑脊液标本中MMP-9含量显著高于对照组，差异有统计学意义（P〈0．05）。结论脑损伤时脑脊液中MMP-9的含量有明显改变，在急性期可能参与脑水肿病理过程，影响脑损伤临床治疗和转归。Objective To investigate the changes of matrix Metalloproteinases-9 （MMP-9） content in the cerebrospinal fluid of patients with brain injury and its clinical significance. Methods Sixty cerebrospinal fluid （CSF） specimens from patients with traumatic brain injury were chosen in our study as experimental group, and other CSF samples from 35 healthy subjects were selected as control group. The content of MMP-9 in cerebrospinal fluid was measured by enzyme-linked immunosorbent assay （ELISA） at 24 h and 72 h, 1 and 2 weeks, respectively; and the comparative study was performed. Results The MMP-9 content in all CSF samples from the experimental group was significantly higher than that in the healthy control group （P〈0.05）. Conclusion MMP-9 content in CSF of patients with brain injury increases obviously; MMP-9 in CSF may contribute to the severity of traumatic brain injury determination and guide the treatment strategies, which is worth for further study.

关 键 词：脑损伤 脑脊液 基质金属蛋白酶-9 

分 类 号：R651.15[医药卫生—外科学]