甘草次酸脂质体体外对鸡IL-2、IL-4和IFN-γmRNA表达的影响  

Effect of glycyrrhetinic acid liposome on mRNA expression of IL-2,IL-4 and IFN-γ in chicken

作　　者：王德青[1,2] 王梦琳[2] 赵晓娟[2] 李彬[2] 赵翰飞[2] 胡元亮[2] 王德云[2] 

机构地区：[1]黄山学院生命与环境科学学院,安徽黄山245041 [2]南京农业大学中兽医学研究室,江苏南京210095

出　　处：《中国兽医学报》2013年第6期874-877,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30901085);教育部科学技术重点项目(108153);中央高校基本科研业务费专项资金项目(KYZ201117)

摘　　要：为了研究甘草次酸脂质体(GAL)增强免疫的作用和分子机理。采用Primer Primier 5.0软件自行设计白细胞介素-2(IL-2)、白细胞介素-4(IL-4)和干扰素-γ(IFN-γ)3对引物。培养鸡外周血淋巴细胞,分别加入3种浓度的GAL、甘草次酸(GA)和空白脂质体(BL),另设LPS对照组,培养36h后收集细胞,提取总RNA,然后对其进行反转录。用荧光定量PCR方法测定GAL对鸡外周血淋巴细胞IL-2、IL-4和IFN-γ的mRNA表达的影响。结果:在8.750mg/L、2.188mg/L、0.547mg/L时,GAL促进外周血淋巴细胞3种细胞因子mRNA的转录能力显著强于GA和BL,且在8.750mg/L时的作用最强。这可能是GAL增强机体免疫的分子机制之一。In order to study the action mechanism of glycyrrhetinic acid liposome （GAL） on immu- nological adjuvant activity. The primers of interleukin-2 （IL-2）,interleukin-4 （IL-4） and interfer- on-y （IFN-7） were designed with Primer 5.0. The chicken peripheral lymphocyte was cultivated, adding GAL,GA and BL at three concentrations respectively. After cultured 36 h, the cells were collected and total RNA was extracted. The transcription of IL-2,IL-4 and IFN-7 mRNA was de- termined by fluorescence quantitative RT-PCR assay. The results showed that GAL could improve the transcription level of IL-2.IL-4 and IFN-7 mRNA,and were significantly higher than GA and BL at 8. 750 mg/L and 2. 188 mg/L. The immune enhancement mechanisms of GAL could be as- sociated to promoting immunity.

关 键 词：甘草次酸脂质体 IL-2 IL-4 IFN-Γ MRNA表达 

分 类 号：S852.4[农业科学—基础兽医学]