纳米磷酸三钙/明胶/鹿茸多肽复合材料的制备及对人成骨细胞增殖、分化及矿化的影响  

Preparation of nano b-tricalcuim phosphate,gelatin and velvet antler polypeptide composites and its effect on the proliferation,differentiation and mineralization in hFOB1.19 cells

作　　者：刘晓峰[1,2] 张郑瑶[1,2] 田秀丽[1] 张瑾[1] 史祺云[1] 胡进平[1] 郭燕川[3] 邓旭明[1] 周秋丽[2] 宋宇[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学药学院,吉林长春130062 [3]中国科学院理化技术研究所,北京100010

出　　处：《中国兽医学报》2013年第6期898-904,共7页Chinese Journal of Veterinary Science

基　　金：国家863计划资助项目(2006AA03Z328);长春市科技局科技支撑计划项目(长科技合2008217号)

摘　　要：探讨反相微乳液法制备的β-纳米磷酸三钙/明胶/鹿茸多肽复合材料(简称纳米复合材料)对人成骨细胞功能的影响。利用反相微乳液法制备纳米复合材料,扫描电镜观察其形态,EDX与SELDI-TOF-MS分析对复合材料进行表征。采用MTT比色法、茜素红染色法检测纳米复合材料对体外培养的人成骨细胞hFOB1.19的增殖、矿化结节形成的影响;采用全自动生化分析仪检测hFOB细胞碱性磷酸酶活性(ALP)。结果表明该纳米复合材料可促进人成骨细胞增殖,提高ALP活性并增加矿化结节形成的数量,可促进人成骨细胞的增殖、分化成熟及矿化。The nano tricalcium phosphate/gelatin/velvet antler polypeptide （VAP）composite ma- terials （referred as nanocomposites） were prepared by water-in-oil （W/O） emulsion process. The effect of nanocomposites on human osteoblasts was studied in hFOB1.19 cell lines. Nanocompos- ites were prepared through emulsification-coacervation method in water-in-oil emulsions. The mor- phology was observed by scanning electron microscope. Characterization of composite spheres was examined by EDX and SELDI-TOF-MS. MTT assay and alizarin red staining were employed to de- tect the effects of nanocomposites on the proliferation and formation of mineralization nodules for- mation in hFOB cells, respectively. The alkaline phosphatase（ALP） activity was determined by au- tomatic clinical chemistry analyzer. The results showed that nanocomposites could stimulate hu- man osteoblasts cells proliferation increase ALP activity,and promote the formation of mineraliza- tion nodules.

关 键 词：纳米磷酸三钙 明胶 鹿茸多肽复合材料 成骨细胞 增殖 分化 矿化 

分 类 号：R285.5[医药卫生—中药学]