基于多重PCR和多重酶切检测猪主要肉质基因多态性的体系构建  

Polymorphisms detection of pork quality genes based on multiplex PCR and multiple enzyme digestion system

作　　者：高峰[1] 印崇[1] 孙博兴[1] 白春艳[1] 徐晓芳[1] 张晓军[1] 张成才[1] 赵志辉[1] 邢沈阳[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2013年第6期923-926,共4页Chinese Journal of Veterinary Science

基　　金：吉林省科技厅农业处资助项目(20120224)

摘　　要：猪的肉质属于数量性状是受多个主效基因和候选基因调控,氟烷基因、酸肉基因和H-FABP基因都是影响猪肉质的主效基因。为了探索在群体中这几个基因多态性快速检测的方法,本研究构建了一种基于双重PCR和双重酶切手段的检测体系,该体系可以快速准确的实现同时扩增和酶切两段DNA片段,提高了检测猪肉质基因多态性的效率。该方法在猪的分子标记辅助选择育种基因型检测中具有一定的应用价值。Pork quality is controlled by several major gene and candidate genes. Halothane,Rende ment Napole and HFABP genes are high correlation to pork quality. In order to detect the polymorphism of halothane,Rendement Napole and HFABP gene swiftly, this research constructed a detection system based on doublePCR and doubleenzyme digestion technique. The system can an alyze the genotype of two mutation site at one time, greatly enhanced the efficient of detecting genes related to pork quality. It has a certain value in the genotype detection of molecular marker assisted selection in swine breeding.

关 键 词：猪 氟烷基因 酸肉基因 H-FABP基因 

分 类 号：S828[农业科学—畜牧学]