基于Nafion共固定Pt/PEDOT/MWCNTs复合材料和辣根过氧化物酶电化学H_2O_2生物传感器  

Electrochemical H_2O_2 Biosensors Based on the Co-immobilization of Pt/PEDOT/MWCNTs Composite and Horseradish Peroxidase with Nafion

作　　者：王珊珊[1] 樊友军[1] 张艳勤[1] 谭一良[1] 钟静萍[1] 王辉[1] 沈星灿[1] 

机构地区：[1]药用资源化学与药物分子工程教育部重点实验室,广西师范大学化学化工学院,广西桂林541004

出　　处：《分析科学学报》2013年第3期327-332,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.21263002,21161003);广西自然科学基金(No.0991093);广西教育厅科研项目(No.201012MS024);广西自然科学基金创新团队项目(No.2010GXNSFF013001);药用资源化学与药物分子工程教育部重点实验室资助课题(No.CMEMR2012-A3)

摘　　要：以多壁碳纳米管(MWCNTs)为载体,采用改进的一步化学合成法制备了Pt/聚3,4-乙烯二氧噻吩(PEDOT)/MWCNTs复合材料,以Nafion共固定此复合材料和辣根过氧化物酶(HRP)从而研制出性能优良的生物传感器。利用扫描电子显微镜(SEM)、傅立叶变换红外(FTIR)光谱和紫外-可见(UV-Vis)吸收光谱对制得的复合材料进行表征,同时通过循环伏安法详细探讨了该传感器对H2O2的响应性能。结果表明,固定的HRP仍能维持其结构和生物活性,并进行有效和稳定的直接电子转移。该方法由于使用了载体MWCNTs及NaBH4的进一步还原过程,明显增强了传感器对H2O2的电催化活性。在优化条件下,该传感器对H2O2在0.008～1.8mmol/L浓度范围内成线性相关,检出限为0.4μmol/L,且具有良好的重现性、稳定性和选择性。A hydrogen peroxide biosensor with high performance was obtained by co-immobilizing horseradish peroxidase（HRP）and the Pt/poly（3,4-ethylenedioxythiophene）（PEDOT）/MWCNTs composite with Nafion,which was prepared through improved one-step chemical synthesis route with multi-walled carbon nanotubes（MWCNTs）as the carriers.The prepared composite was characterized by scanning electron microscope（SEM）,Fourier transform infrared（FTIR）spectroscopy and ultraviolet visible（UV-Vis）absorption spectroscopy.The response properties of the biosensor to H2O2 were discussed in detail by cyclic voltammetry,and its preparation conditions were optimized.The results demonstrated that the immobilized HRP could maintain its native structure and bioactivity,and effectively process the direct electron transfer.Because the MWCNTs carrier and reduction process of NaBH4 were used in this method,the electrocatalytic activity of the biosensor to H2O2 was significantly enhanced.Under the optimum conditions,the response currents had good linear relationship with H2O2 concentration in the range of 0.008-1.8 mmol/L with a detection limit of 0.4μmol/L,as well as excellent reproducibility,stability and selectivity.

关 键 词：电化学H2O2生物传感器 多壁碳纳米管 纳米铂 聚3 4-乙烯二氧噻吩 辣根过氧化物酶 

分 类 号：O657.15[理学—分析化学]