高效液相色谱-电感耦合等离子体质谱分析几种富硒产品的硒总量及其形态  

Determination of Total Se and Selenium Speciation in Enriched Products by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry

作　　者：仲娜 李新荣 仲丽 周静洋[3] 王景禄 杨晓云 

机构地区：[1]青岛市药品检验所,山东青岛266071 [2]山东省城市服务技术学院,山东烟台264670 [3]山东中医药大学,山东济南250014

出　　处：《分析科学学报》2013年第3期435-438,共4页Journal of Analytical Science

摘　　要：本文采用高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)分析了几种富硒产品中硒的总量及其形态。结果表明,四种富硒产品的硒总量均有不同程度增加,富硒大米和富硒茶叶中的硒总量较高,分别为1.300μg/g和0.459μg/g,富硒大米和富硒大葱的富硒倍率相对较高,分别为未富硒产品的24.1和17.8倍。以0.1mol/LHCl辅助超声预处理样品进行富硒螺旋藻和富硒大葱中硒的形态分析,发现富硒大葱中的硒主要为有机态的甲基硒代半胱氨酸(MeSeCys)以及部分硒代蛋氨酸(SeMet),而富硒螺旋藻中的硒大部分为无机硒。说明经根部吸收等富集方式可有效将无机硒转化为有机硒,而某些方式未能完全转化。In this work,total Se and Se speciation in the Se-enriched products were determined by high prformance liquid chromatography-inductively coupled plasma-mass spectrometry.The result showed that total Se in four Se-enriched products were increased to some extent,and Se-enriched rice and tea have the higher contents of 1.300μg/g and 0.459μg/g,while Se-enriched rice and green onion have the higher enriched rates of 24.1and 17.8times,respectively.Se speciation analysis results showed that Se exists as two main organic selenium species in Se-enriched green onion,while exists as inorganic selenium species in Se-enriched spirulina,which indicates that some Se-enriched pathway could effectively transfer inorganic selenium to organic selenium.

关 键 词：富硒产品 高效液相色谱-电感耦合等离子体质谱 总硒 形态分析 

分 类 号：O657.63[理学—分析化学]