从核心蛋白聚糖和基质金属蛋白酶-1探讨电针对压力性尿失禁大鼠盆底组织胶原蛋白代谢的影响  

Investigating the Effect of Electroacupuncture on Collagen Metabolism in the Pelvic Floor Tissue of Stress Incontinence Rats from Decorin and Matrix Metalloproteinases-1

作　　者：张淑静[1] 于芳 汪司右[1] 

机构地区：[1]上海市针灸经络研究所,上海200030 [2]河北省保定易县中医院,河北074200

出　　处：《上海针灸杂志》2013年第6期520-522,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：上海市科学委员会科研项目(12401904600);上海市重点学科项目(JZ2012004)

摘　　要：目的通过观察压力性尿失禁(SUI)大鼠盆底支持组织核心蛋白聚糖(DCN)和基质金属蛋白酶-1(MMP-1)的变化,探讨电针对SUI大鼠盆底支持组织胶原蛋白代谢影响。方法通过阴道扩张和双侧卵巢切除建立大鼠SUI动物模型。采用实时定量PCR法观察尿道下阴道壁、耻骨尿道韧带、耻骨尾骨肌和尿道周围结缔组织中DCN和MMP-1 mRNA的表达。结果电针可显著降低SUI大鼠盆底支持组织DCN和MMP-1 mRNA的表达(与模型组比,P<0.05),其中对MMP-1的影响较为明显。结论电针对SUI大鼠盆底支持组织DCN和MMP-1蛋白的下调作用可能是针灸治疗SUI的机制之一。Objective To investigate the effect of electroacupuncture on collagen metabolism in the pelvic floor supporting tissue by observing decorin （DCN） and matrix metalloproteinases-1 （MMP-1） in the pelvic floor tissue of stress urinary incontinence （SUI） rats. Method A rat model of SUI was made by colpeurysis and bilateral ovariectomy. The expressions of DCN and MMP-1 mRNA in the suburethral vaginal wall, pubourethral ligament, pubococcygeus and periurethral connective tissue were examined by real-time quantitative PCR. Result Electroacupuncture decreased significantly the expressions of DCN and MMP-1 mRNA in the pelvic floor supporting tissue of SUI rats （in comparison with the model group, P〈0.05） with a more marked effect on MMP-1. Conclusion The down-regulating effect of electroacupuncture on DCN and MMP-1 in the pelvic floor supporting tissue of stress incontinence rats may be one of the mechanisms for acupuncture treatment of stress incontinence.

关 键 词：电针 大鼠 尿失禁 核心蛋白聚糖 基质金属蛋白酶-1 

分 类 号：R2-03[医药卫生—中医学]