水杨酸甲酯乳糖苷有关物质测定方法的研究  

Study on the related substances of methyl salicylate-2-O-β-Dgalactopyranoside(1-4)-β-D-glucopyranoside by HPLC

作　　者：何秀峰[1] 夏鹏飞[1] 王爱国[1] 赵立敏[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所北京协和药厂,北京100050

出　　处：《中国新药杂志》2013年第11期1259-1262,共4页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX102-34)

摘　　要：目的:建立水杨酸甲酯乳糖苷有关物质的高效液相色谱测定方法。方法:液相色谱条件:phe-nomnex氰基色谱柱(250 mm×4.6 mm,5μm);以乙腈-水为流动相梯度洗脱,流速为1.0 mL.min-1,检测波长为286 nm,柱温为40℃;质谱条件:电喷雾电离源检测;源温80℃。结果:检测出水杨酸甲酯乳糖苷原料经酸、碱、高温、强光、氧化破坏后得到的4个有关物质(1～4),4个有关物质与主峰得到很好的分离。结论:本实验采用液质联用法,灵敏度高,分离度好,能用于水杨酸甲酯乳糖苷的质量控制。Objective：To establish an HPLC method for determination of related substances of the methyl salicylate glycoside （MSG）.Methods：Phenomnes CN column（250 mm×4.6 mm,5 μm）was applied; the mobile phase was acetoni-water gradient elution, the flow rate was 1.0 mL·min-1; the detection wavelength was 286 nm; the column temperature was 40 ℃. Melectrospray ionization was used in MS and the source temperature was 80 ℃. Results：Four related substances（1~4）has been detected in the MSG substance by acid, base, high temperature, highlight and oxidation destroy. Four related substances and peak were well separated. Conclusion：The method is of high sensitivity and good separation, which could be used in the quality control of MSG.

关 键 词：水杨酸甲酯乳糖苷 有关物质 高效液相色谱法 液质联用 

分 类 号：R917[医药卫生—药物分析学] R971.1[医药卫生—药学]