葛根素对高脂联合STZ诱导糖尿病肾病大鼠肾组织纤维连接蛋白与IV型胶原蛋白表达的影响  

Effect of puerarin on renal expression of fibronectin and type IV collagen proteins in rats with diabetic nephropathy induced by combination of high-fat diet and low-dose streptozotocin

作　　者：唐丽琴[1] 杨峰[2] 王凤玲[2] 朱玲娜[3] 蔡明[3] 魏伟[2] 

机构地区：[1]安徽医科大学附属省立医院,合肥230001 [2]安徽医科大学临床药理研究所,合肥230032 [3]安徽中医学院,合肥230031

出　　处：《中国新药杂志》2013年第11期1320-1324,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金(81073109;81102864);安徽省自然科学基金(090413106);安徽省卫生厅医学科研课题(09C219)

摘　　要：目的:探讨葛根素对糖尿病肾病(DN)大鼠肾病变的保护作用及其机制。方法:以高脂饲料喂养联合低剂量链脲佐菌素(STZ)复制DN大鼠模型,动物分为正常对照组、模型组、3个葛根素治疗组、依那普利治疗组、消渴丸治疗组。8周后检测空腹血糖、尿素氮、血肌酐(SCr)、微量白蛋白/尿肌酐比值(U.A/C)和总蛋白/尿肌酐比值(UTP/C)等;Masson染色法观察肾组织形态学改变;免疫组织化学检测肾组织纤维连接蛋白(FN)与IV胶原蛋白(IV-C)的表达。结果:ig给药,葛根素可改善DN大鼠肾脏功能和肾组织形态学异常,降低肾组织中FN与IV-C的表达。结论:葛根素对DN大鼠肾脏具有保护作用,此作用可能与下调肾脏组织FN与IV-C的表达有关。Objective： To investigate the effect of puerarin on diabetic nephropathy and the mechanism in rats. Methods： The rat model of diabetic nephropathy was induced by intraperitoneal injection of low-dose of streptozocin （STZ） after having high-fat diet for six weeks. The rats were treated with 3 doses of puerarin, enalapril or XiaoKe Wan every day. At 8 weeks after treatment, biochemical indicators were evaluated. The pathological changes in the kidney were determined by Masson staining. The expression of fibronectin （FN） and type IV collagen （IV-C） were detected by immunohistochemistry. Results： Puerarin suppressed histological changes in the kidney tissues, and reduced the levels of these biochemical parameters and the expression of FN and IV-C proteins in renal tissues of diabetic rats with nephropathy. Conclusion： Puerarin protects renal function and slows down the progression of diabetic nephropathy in rats by suppressing the expression of FN and IV-C proteins in renal tissue.

关 键 词：葛根素 糖尿病肾病 纤维连接蛋白 IV胶原蛋白 

分 类 号：R961[医药卫生—药理学]