基于GSH-CdTe/CdS量子点的荧光变化研究hsDNA与盐酸洛美沙星-Cu(Ⅱ)配合物的相互作用  

Interaction of Herring Sperm DNA with Lomefloxacin Hydrochloride-Cu(Ⅱ) Based on Changes in the Fluorescence Intensity of GSH-CdTe/CdS Quantum Dots

作　　者：沈益忠[1] 刘绍璞[1] 殷鹏飞[1] 何佑秋[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《高等学校化学学报》2013年第6期1360-1367,共8页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20875078)资助

摘　　要：采用水相法合成了谷胱甘肽(GSH)修饰的CdTe/CdS量子点(GSH-CdTe/CdS QDs).透射电子显微镜表征结果表明,GSH-CdTe/CdS QDs的粒径分布均匀,分散性好.在Tris-HCl(pH=7.6)缓冲液中,由于静电引力作用,带正电的盐酸洛美沙星(LMFH)-Cu(Ⅱ)配合物[LMFH-Cu(Ⅱ)]吸附到带负电的GSH-CdTe/CdSQDs表面形成基态复合物,导致GSH-CdTe/CdS QDs的荧光猝灭.随后,向GSH-CdTe/CdS QDs-LMFH-Cu(Ⅱ)配合物体系中加入鲱鱼精DNA(hsDNA),hsDNA可诱导LMFH-Cu(Ⅱ)配合物从GSH-CdTe/CdS QDs表面脱落而嵌入到hsDNA的双螺旋结构中,使GSH-CdTe/CdS QDs的荧光恢复.通过对GSH-CdTe/CdS QDs荧光的可逆调控,利用荧光光谱、紫外-可见吸收光谱和共振瑞利散射光谱研究了hsDNA与LMFH-Cu(Ⅱ)配合物的相互作用.通过对比GSH-CdTe/CdS QDs与LMFH相互作用的光谱性质,讨论了GSH-CdTe/CdSQDs-LMFH-Cu(Ⅱ)-hsDNA的相互作用机理,模拟了作用过程,从而建立了一种研究氟诺喹酮类药物的金属配合物与核酸相互作用机制的光谱方法.Glutathione（GSH）-capped CdTe/CdS quantum dots（GSH-CdTe/CdS QDs） were synthesized in aqueous solution.The particle sizes and morphological characteristics of GSH-CdTe/CdS QDs were investigated by transmission electron microscopy（TEM）.The results exhibits that the particle size of as-prepared QDs has a narrow size distribution and good dispersivity.In Tris-HCl buffer medium（pH=7.6）,lomefloxacin hydrochloride-Cu（Ⅱ） coordination compound（LMFH-Cu2＋） was adsorbed to the surfaces of GSH-CdTe/CdS QDs through electrostatic attraction and formed ground state complex,which resulted in the quenching of the fluorescence of GSH-CdTe/CdS QDs.Adding herring sperm DNA（hsDNA） to GSH-CdTe/CdS QDs-LMFH-Cu（Ⅱ） system led to the fluorescence intensity of GSH-CdTe/CdS QDs recover,which can be explained by that the addition of hsDNA to the system induced LMFH-Cu（Ⅱ） to dissociate from the surface of GSH-CdTe/CdS QDs and embed into its double helix structure.According to the fluorescence quenching and restoration for GSH-CdTe/CdS QDs,fluorescence reversible control of GSH-CdTe/CdS QDs was realized.Compared with the interaction between GSH-CdTe/CdS QDs and LMFH,the interaction of GSH-CdTe/CdS QDs-LMFH-Cu（Ⅱ）-hsDNA was studied by fluorescence（FL）,resonance Rayleigh scattering（RRS） and ultraviolet-visible absorption（UV-Vis） spectra.Meanwhile,the interaction mechanism was discussed and corresponding model of interaction was built.

关 键 词：GSH-CdTe CdS量子点 盐酸洛美沙星-Cu(Ⅱ) 鲱鱼精DNA 荧光可逆调控 

分 类 号：O657[理学—分析化学]