东北地区香茶菜属植物遗传多样性的RAPD分析  

RAPD Analysis on the Genetic Diversity of Genus Rabdosia in Northeast China

作　　者：张博[1] 刘翠晶[1] 刘霞[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《北方园艺》2013年第12期87-90,共4页Northern Horticulture

摘　　要：以东北地区不同产地的24份蓝萼香茶菜和尾叶香茶菜样品为试材,采用RAPD分子标记技术对其进行遗传多样性分析。结果表明:从60条随机引物中筛选出5条引物用于2种香茶菜的扩增,2种香茶菜共扩增得到63条清晰条带,其中55条为多态性条带,多态性比率为87.30%。系统聚类分析结果表明,2种香茶菜亲缘关系明显,遗传多样性较丰富,且种间大于种内。因此,RAPD分子标记技术可以作为研究香茶菜属植物遗传多样性的有效标记方法。Taking 24 samples of Rabdosia japonica（Burm.f.） Hara var.glaucocalyx（Maxim.） Hara and Rabdosia excise（Maxim.） Hara from different regions of northeast China as materials,the genetic diversity was studied using random amplified polymorphism DNA（RAPD） markers.The results showed that 5 primers were selected from 60 primers.63 DNA bands were amplified from the two species of Rabdosia,the percentage of polymorphism was 87.30%.Analysis of Nei＇s unbiased genetic distance showed that the genetic relationship between the two species of Rabdosia was significant.The genetic diversity was high,and the genetic diversity of interspecies was higher than that of interspecies.In conclusion,RAPD molecular marker technology could be an efficient method to study the genetic diversity of Rabdosia plants.

关 键 词：蓝萼香茶菜 尾叶香茶菜 RAPD 聚类分析 

分 类 号：Q949.95[生物学—植物学]