月季“圣斯威辛”的快速繁殖技术研究  

Study on the Rapid Propagation of Rosa 'St.Stwithum'

作　　者：徐玲玲[1] 党悦方[1] 陶贵荣[1] 裴昕 郭斌[3] 

机构地区：[1]西安文理学院生物技术学院,陕西西安710065 [2]西安芳田玫瑰农业科技有限公司,陕西西安710061 [3]西北大学生命科学学院,西部资源生物与现代生物技术教育部重点实验室,陕西西安710069

出　　处：《北方园艺》2013年第12期97-99,共3页Northern Horticulture

基　　金：国家自然科学基金资助项目(31000144);陕西省自然科学基础研究计划资助项目(2012JQ3017);西安市科技计划资助项目(NC1206(3));陕西省教育厅基金资助项目(12JK0846)

摘　　要：以"圣斯威辛"(Rosa‘St.Stwithum’)月季带腋芽的节为外植体,研究了"圣斯威辛"月季不定芽试管生根的最佳条件。结果表明:0.05mg/L NAA和3mg/L 6-BA的MS培养基中月季不定芽的诱导频率和平均每个外植体上的芽数最多,分别为95.7%和8.4个。将生长至3cm的不定芽转移到生根培养基上诱导根的形成,无激素处理的月季组培苗的平均每个不定芽生根数为6.3条,根诱导频率92%,平均根长为3.7cm。该结果与添加低浓度的IBA(0.2mg/L)对根的诱导效果没有显著性差异。高浓度的NAA和IBA诱导出大量的愈伤组织,不定根的数量显著减少。将生根的月季试管苗移栽到温室中,移栽成活率达到92%。Taking the axillary buds of Rosa ＇St.Stwithum＇ as explants,an efficient micropropagation system for Rosa ＇St.Stwithum＇ were studied.The results showed that 95.7% adventitious buds induction frequency and 8.4 buds per explant were achieved when cultured on MS media containing 0.05 mg/L NAA and 3 mg/L 6-BA.After transferred the adventitious buds,which length had grown to 3 cm,to rooting media,root organogenesis occurred.The control without any exogenous auxins treatment,which average roots number per stem,induction frequency and the average roots＇ length were 6.3 roots,92% and 3.7 cm,respectively,had no significant differences with 0.2 mg/L IBA treatments.High concentrations of NAA and IBA treatments induced a lot of callus,and significantly decreased adventitious roots.92% of the regenerated plantlets survived and grew vigorously in greenhouse condition.

关 键 词：“圣斯威辛”月季 组织培养 不定芽 NAA IBA 

分 类 号：S685.12[农业科学—观赏园艺]