竹节参对MC3T3-E1成骨样细胞增殖与分化的影响  

Effects of Traditional Chinese Medicine Panax Japonicus on Promoting the Proliferation,Differentiation of MC3T3-E1 Cells

作　　者：朱盼盼[1] 李飞[1] 陈平[2] 杨中林[1] 

机构地区：[1]中国药科大学天然药物活性物质与功能国家重点实验室,江苏南京210009 [2]武汉轻工大学生物与制药工程学院,湖北武汉430023

出　　处：《亚太传统医药》2013年第6期11-13,共3页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金项目(81274023)

摘　　要：目的:研究竹节参不同提取物对体外培养成骨样细胞(MC3T3-E1)增殖、分化作用的影响。方法:采用MC3T3-E1细胞为体外药物筛选的细胞模型,用MTT法测定药物对成骨细胞的增殖作用,碱性磷酸酶(ALP)试剂盒测定ALP的活性,考察竹节参不同提取物对MC3T3-E1细胞增殖、分化作用的影响。结果:10-1 mg/mL的竹节参水提物和10-4 mg/mL的95%乙醇提取物能显著促进MC3T3-E1细胞的增殖和分化(P<0.05)。结论:一定浓度的竹节参水提物和95%乙醇提取物能显著促进MC3T3-E1细胞的增殖和分化,该药物具有开发抗骨质疏松药物的潜力。Objective：To study the effects of different extracts of Panax japonieus on the proliferation and differentiation of osteoblastic MC3T3-E1 cells. Methods： Osteoblast cell line MC3T3-E1 was used as a cell model for screening potency of Panax japoni cus. MTT was applied to determine the proliferation promoted by aqueous and 95% ethanol extracts at different doses in MC3T3-E1 cells. Differentiating effects of the two extracts with different concentrations were also evaluated through the examinations of alkali phosphate （ALP） activities. Results ： The 10^-1 mg/mL of the aqueous extracts and 10 ^-4mg/mL of 95 % ethanol extracts could significantly promote the proliferation and differentiation of MC3T3-E1 cells. Conclusion： A certain concentration of aqueous extracts and 95 % ethanol extracts of Panax japonicus could significantly promote the proliferation and differentiation of MC3T3-EI. This herb could be developed into a new drug for treatment of osteoporosis .

关 键 词：竹节参 MC3T3-E1 增殖 分化 

分 类 号：R284.1[医药卫生—中药学]