山绿茶提取物对体外人血小板聚集的影响  

Effect of Extracts from Ilex Hainanensis Merr. on Human Platelet Aggregation in Vitro

作　　者：李浩野[1,2] 孟兰贞[1,2] 黄卫华[1,2] 赵静[1,2] 刘华钢[3] 李绍平[1,2] 

机构地区：[1]澳门大学 中药质量研究国家重点实验室,澳门999078 [2]澳门大学中华医药研究院,澳门999078 [3]广西医科大学,广西南宁530021

出　　处：《药物生物技术》2013年第3期238-240,共3页Pharmaceutical Biotechnology

基　　金：国家自然科学基金(No.30850010);澳门大学基金项目(No.UL015A)

摘　　要：山绿茶为广西少数民族民间中草药,具有活血通脉等治疗心血管疾病的功效。为观察山绿茶提取物对花生四烯酸钠盐(AA)、二磷酸腺苷(ADP)、盐酸肾上腺素(ADR)诱导的体外人血小板聚集的影响,通过阿司匹林为阳性药建立体外抗人血小板聚集模型,以健康人富血小板血浆为研究对象,分别以AA、ADP、ADR作为诱导剂,运用Born氏比浊法测定体外诱导的人血小板聚集率,探究不同山绿茶提取物对体外诱导的人抗血小板聚集的影响。实验结果表明:阳性药阿司匹林对诱导的体外人血小板聚集有明显抑制作用,而山绿茶各提取物对诱导的体外人血小板聚集无显著影响,虽然山绿茶提取物对体外人血小板聚集的抑制作用为阴性结果,但为地方中药材标准的完善提供了实验依据。llex hainanensis Men. is the national minority folk herbal medicine in the Guangxi Zhuang Autonomous Region, and its effects of blood invigorate and pulse promotion can provide potential therapeutic approaches to treatment cardiovascular diseases. The aim of this paper is to observe the effect of different extracts from flex hainanensis Men＂ （IHE） on human platelet aggregation induced by arachidonic acid sodium salt （AA） ,adenosine diphosphate （ADP）, and epinephrine hydrochlaride （ADR） ,respective- ly, in vitro. In this study, aspirin was used as positive control drug to establish the in vitro anti-platelet aggregation model and platelet aggregation rate was measured by Born＇s method. Platelet-rich plasma was collected from healthy donors and induced by AA, ADP and ADR, respectively and the model was applied to study the effect of different IHE on the platelet aggregation. The results showed that aspirin can significantly inhibit platelet aggregation induced by A＇A, ADP and ADR in vitro, respectively, but the IHE did not show inhibiting effect on the human platelet aggregation significantly. However,the IHE negative results can provide solid experimen- tal evidence for improving drug quality standard.

关 键 词：山绿茶 血小板聚集 阿司匹林 花生四烯酸钠盐 二磷酸腺苷 盐酸肾上腺素 人体模型 

分 类 号：R965[医药卫生—药理学]