蛋白组学技术在抗蛇毒血清研究中的运用  

Application of Proteomic Tools in Snake Antivenoms

作　　者：曹宇亮[1] 刘明华[1] 

机构地区：[1]第三军医大学西南医院急救部,四川重庆400038

出　　处：《药物生物技术》2013年第3期262-265,共4页Pharmaceutical Biotechnology

基　　金：国家自然科学基金资助项目(No.8101537);重庆市攻关课题(No.cstc/20105026);第三军医大学临床创新基金资助项目(No.2009xlc15)

摘　　要：毒蛇咬伤是热带、亚热带地区比较常见,给公众健康带来严重危害的一类疾病。动物源性的抗蛇毒血清是现代蛇伤治疗的主要手段,但是由于蛇毒成分复杂,且存在种内、种间变异,由全蛇毒免疫所获得的抗蛇毒血清存在诸多不足之处。随着蛋白组学技术在蛇毒研究的应用,使人们对蛇毒的研究有了一些新的认识,近年来出现的"蛇毒蛋白组学"(Venomics)和"抗蛇毒血清组学"(Antivenomics)技术的出现,为生产新型的、毒素特异性的抗蛇毒血清带来了可能,本文现就"蛇毒蛋白组学"(Venomics)和"抗蛇毒血清组学"(Antivenomics)在蛇毒研究及抗蛇毒血清设计和质量控制等几个方面进行综述。Snakebite envenoming is common in the tropic and subtropics region, it has heavy public health impact. Adequate treat- ment of snakebite envenoming has been critically dependent on the availability of animal-derived antivenoms in the recent years. Because of the complexity of snake venom and the inter-and intraspecies heterogeneity in venom composition, the animal-derived antivenoms hyperimmunized by whole venom possesses some deficiencies. Through the application of the proteomic tools in snake venom, more knowledge about the compositon of snake venom is obtained. Recently, the presence of venomics and antivenomics may bring possibility in the production of the novel toxin-specific antivenoms. This paper reviews the application of the proteomic for designing and quality control of antivenoms.

关 键 词：蛇毒 抗蛇毒血清 蛋白组学 毒蛇咬伤 

分 类 号：Q51[生物学—生物化学]