呋喃唑酮代谢物人工抗原的合成及抗体的制备  

Synthesis of Artificial Antigens and Preparation of Specific Antisera against 3-amino-2-oxazolidinone,a Metabolite of Furazolidone

作　　者：贾慧勤[1] 丁焕中[1] 刘晓云 李小红[1] 张炳旭[1] 鲁晓雄[1] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广州万孚生物技术股份有限公司,广州510641

出　　处：《中国兽药杂志》2013年第6期20-23,共4页Chinese Journal of Veterinary Drug

摘　　要：为改进和完善免疫检测呋喃唑酮代谢物方法,制备了抗呋喃唑酮代谢物(AOZ)特异性抗体。采用对醛基苯甲酸对AOZ进行衍生化得到CPAOZ,还原CPAOZ结构中的C=N双键得到半抗原,半抗原用N-羟基琥珀酰亚胺活化酯法与BSA偶联制备免疫原,免疫新西兰大白兔制得特异性抗体,采用间接(竞争)ELISA法评价抗血清效价及特异性。结果显示,试验获得了较高效价(1∶1280000)的抗AOZ血清,AOZ半抑制浓度为5.9 ng/mL;抗血清与结构类似物呋喃它酮代谢物的交叉反应率仅为0.76%,与呋喃妥因代谢物和呋喃西林代谢物无交叉反应;利用该抗体建立的间接竞争ELISA检测法,AOZ在1～27 ng/mL与抑制率呈线性关系。结果表明,该特抗体虽然灵敏度较低,却对AOZ而非AOZ衍生物有特异性,可为畜产品中AOZ残留检测提供新的思路和方法。In order to prepare specific antibody against 3 - amino - 2 - oxazolidinone （ AOZ）, a metabolite of furazolidone, AOZ was activated by 4 - formylbenzoic acid and then reduced the schiff base, then conjugated with BSA via EDC/NHS as immunogen. Rabbits were immunized with immunogen; antisera were evaluated by indirect （competitive） ELISA （IC -ELISA）. Antisera titer was 1 ： 1280000 and ICs0 was 5.9 ng/mL. The antisera had hardly cross - reaction with AMOZ, AHD and SEM. An IC - ELISA was developed for the determination of AOZ in the linear range of 1 -27 ng/mL. Although the sensitivity is not high, the new antibodies with high specificity against AOZ not AOZ derivatives were obtained in this study which would provide a new thought and method for the detection of AOZ in livestock and poultry products.

关 键 词：呋喃唑酮代谢物 特异性抗体 间接(竞争)ELISA 

分 类 号：S859.84[农业科学—临床兽医学]