基于四交群体鉴定玉米开花期相关性状的QTL  

Identification of QTL for traits related to flowering time in maize based on four-way cross population

作　　者：李永明[1] 李贤唐[1] 陈甲法[1] 周波[1] 周子键[1] 丁俊强[1] 吴建宇[1] 

机构地区：[1]河南农业大学农学院,河南郑州450002

出　　处：《河南农业大学学报》2013年第3期231-240,共10页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(31271307)

摘　　要：为研究玉米开花期的遗传机制,利用4个玉米自交系组配276/72//A188/交51四交群体,构建了包含213个SSR分子标记的遗传连锁图谱.在河南郑州和济源2个环境下共检测到开花期QTL 41个,大部分为微效等位基因,加性效应为主要效应.采用综合农艺性状加分子标记辅助选择的方法,以吐丝期1个QTL位点qSE4a所在的目标区间及其附近区间(umc1511～umc1051,bin4.05～bin4.08)为目标区段构建A188为供体亲本,交51为受体亲本的5个NIL,初步验证了该位点的遗传效应,并初步定位到标记bnlg2291附近.For studying the genetic mechanism of flowering time in maize, a four-way cross population has been used derived from four maize inbred lines with the 276/72//A188/Jiao51 crossing pattern to map quantitative trait loci that influence the traits, and the genetic linkage map was constructed used by 213 SSR （simple sequence repeat） molecular makers. 41 QTLs for the traits related to flowering time were detected under two environments （Henan Zhengzhou, Henan Jiyuan）. Most of those QTL were minor alleles, and the additional effect predominated in the traits. Five NILs of the qSE4a, a QTL controlling the day to silk emergence in maize, were developed by the backcross breeding, used by the inbred A188 as the donor parent with the inbred Jiao51 as the recipient parent, under the mo- lecular makers. The target segment on chromosome 4 （umcl511 - umcl051, bin4.05 - bin4.08） containing the target region where the qSE4a was located and its neighboring region was used to devel- op the NILs. Finally, this locus, which was verified with the genetic effect, was preliminarily mapped nearby the molecular maker bnlg2291.

关 键 词：玉米 四交群体 开花期 数量性状位点 近等基因系 

分 类 号：S513[农业科学—作物学]