7种木兰科常绿植物亲缘关系的ISSR和AFLP分析  

AFLP and ISSR markers analysis of the genetic relationship of 7 kinds of magnoliaceae evergreen plants

作　　者：李剑[1] 靳晓东[1] 杨秋生[1] 裴海潮 刘增喜[2] 

机构地区：[1]河南农业大学林学院,河南郑州450002 [2]河南省经济林和林木种苗工作站,河南郑州450008

出　　处：《河南农业大学学报》2013年第3期289-295,共7页Journal of Henan Agricultural University

基　　金：国家林业局林业公益性行业科研专项(201004031)

摘　　要：利用ISSR和AFLP 2种分子标记方法对7种木兰科常绿植物遗传多态性和亲缘关系进行了分析,建立了木兰科常绿植物ISSR和AFLP的优化反应体系,构建了UPGMA系统树.利用筛选的11个引物对7份样本进行ISSR分析,共获得131条带,其中多态性带86条,平均每个引物产生11.10条带,平均多态性检出率为70.37%,说明供试的7种材料种质间有较高等位多态性.用22对EcoRⅠ/MseⅠ引物组合对7份样本进行了AFLP分析,共扩增出1 310条带,其中多态性带1 204条,平均每对引物扩增带数59条,多态性率92.5%.通过2种标记发现,木兰属与含笑属聚类在一起,他们之间存在的遗传差异通过分子标记可以鉴定出来,而木莲属的川滇木莲在聚类分析中差异性最明显,尤其与含笑属,虽然他们的外部形态相似,但遗传距离较远.研究还发现,在2组试验的遗传相似系数结果中,均有超出0～1范围的数值出现,根据分析为偏分离现象,初步推断出现此种结果应与材料来源有关.To establish optimized reaction system and construct phenetic trees, we analysed the genetic polymorphisms and relationship of 7 kinds of magnoliaceae evergreen plants with AFLP and ISSR mark- ers. 7 kinds of materials were analysed with 11 screened primers by ISSR and 131 bands were ob- tained, and 86 of them were polymorphic bands, each primer producing 11.10 bands, with an average polymorphism detection rate of 70.37% , which indicated that the polymorphism between those plant materials germplasm was very high. And then the 7 kinds of materials were analysed with 22 pairs of EcoR I/Mse I primer combinations by AFLP, 1 310 bands, and 1 204 of them were polymorphism bands,each pair of primer producing 59 bands, and the polymorphism rate was 92.5%. The results of these two molecular markers showed that plants in Magnolia and Michelia were clustered together, the genetic differences between them could be identified by molecular markers, however, the Manglietia duclouxii in Magnoliaceae showed the most significant differences in clustering analysis, especially compared with Michelia, although their external form were similar, the genetic distance was farther. The study also found that among these two groups of genetic similarity coefficient, there were somenumbers beyond the range of 0 to 1. This phenomenon was partial separation, and it is preliminarily infered that it was related with the sources of plant materials.

关 键 词：遗传多样性 ISSR AFLP 木兰科 常绿植物 

分 类 号：S682.31[农业科学—观赏园艺]