舟山群岛四个养殖獐种群遗传多样性和遗传结构  

Genetic diversity and genetic structure of four cultured populations of Chinese water deer (Hydropotes inermis) in Zhoushan Archipelago

作　　者：林杰君[1] 鲍毅新[1] 刘军[1] 王艳妮[1] 张旭[1] 

机构地区：[1]浙江师范大学生态研究所,金华321004

出　　处：《生态学报》2013年第11期3460-3469,共10页Acta Ecologica Sinica

基　　金：浙江省研究生创新科研项目(YK2009038);浙江省教育厅资助项目(Y201016779)

摘　　要：基于ISSR分子标记技术,对来自舟山群岛4个獐(Hydropotes inermis)养殖种群的遗传多样性和遗传结构进行了分析。26条ISSR引物共扩增出286个可分析位点,多态位点百分比(PPL)为64.34%。獐物种水平上的Nei's遗传多样性指数(H)为0.210,Shannon's多态信息指数(I)为0.318,各种群的H介于0.157—0.190之间,I在0.228—0.278之间,与已报道的ISSR标记在其它动物物种中的应用结果相比,其遗传多样性较为丰富。Structure软件分析结果显示,所有个体根据遗传信息的不同可以被分为4个组群,每个养殖场内的个体基本上都在各自独立的组群中,这与地理区域的划分相似。另外发现各养殖场獐种群间已表现出了较大的遗传分化(Gst=0.163)。同时,为了解各种群间的遗传关系,计算了各种群间的Nei's遗传距离,结果显示舟山种群(ZS)和秀山种群(XS)之间的遗传距离最近,为0.045,而岱山种群(DS)和朱家尖种群(ZJJ)间的遗传距离最大,为0.066。基于以上结果,建议加强不同岛屿种群间的个体交流,特别是朱家尖种群(ZJJ)与其它种群间的交流。Inter-simple sequence repeats（ISSR） were used to analyze genetic diversity and genetic structure of four cultured populations of Hydropotes inermis in four farms of Zhoushan Archipelago.Twenty-six ISSR primers yielded 286 loci in 59 individuals from the four populations.Among which 184 loci were polymorphic,and percentage of polymorphic loci（PPL） was 64.34%.Primer UBC 812 revealed the highest variation with PPL（90.91%）,whereas primer UBC 860 showed the lowest variation with PPL（37.50%）.Twenty-six ISSR primers were selected for the analyses of genetic diversity and genetic structure in the four population.Nei′s genetic diversity（H） and Shannon′s index（I） at the species level were 0.210 and 0.318,respectively.H values were between 0.1571 and 0.1895 for the four populations of Hydropotes inermis,whereas I values for the four populations were between 0.2277 and 0.2778.When comparing with the results from other animals using ISSR,the genetic diversity of the cultured Hydropotes inermis in Zhoushan Archipelago is relatively high.Among the four populations,Zhoushan（ZS） population showed the highest genetic diversity（Na=1.486,Ne=1.333,H =0.190,I=0.278,PPL=48.60%）,followed by Daishan（DS） population and Xiushan（XS） population.The lowest genetic diversity was found in Zhujiajian（ZJJ） population.The analyses of population genetic structure showed that when K=4,the △K value reached maximum,which suggests that all individuals could be divided into four groups based on their genetic data.Biological data,such as skin color collected from the four populations showed that most individuals from four geographical locations could be clustered into its own group.The color of the majority of DS population was white;the color of the majority of XS population was dark grey;the color of the majority of ZJJ population was black;and the color of the majority of ZS population was light grey.These biological data indicate that there are genetic differences among the four populations studied.Fu

关 键 词：舟山群岛 獐 ISSR 遗传多样性 遗传结构 

分 类 号：S865[农业科学—野生动物驯养]