止消通脉宁对KKAy小鼠肾纤维化及MMP-2/TIMP-1mRNA表达的影响  

The Influences of Zhixiaotongmaining on the Kidney TIMP-1/MMP-2 mRNA Expression in KKay Mice With Diabetic Nephropathy

作　　者：闫小光[1] 孙文[1] 刘铜华[1] 吴丽丽[1] Peter Margetts 敬珊珊[1] 郭翔宇[1] 杨丽霞[3] 徐暾海[1] 周静鑫[1] 艾志敏[1] 

机构地区：[1]北京中医药大学,北京100075 [2]McMaster University [3]甘肃省中医药研究院,甘肃730020

出　　处：《中国中西医结合肾病杂志》2013年第5期409-412,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：科技部国际科技合作项目(No.2009DFA31520);北京中医药大学创新团队项目(No.2011-CXTD-19)

摘　　要：目的:探讨止消通脉宁对自发性2型糖尿病KKAy小鼠肾纤维化及TIMP-1/MMP-2mRNA表达的影响。方法:将SPF级16周龄雄性KKAy小鼠50只按血糖值随机分为模型组、缬沙坦组、止消通脉宁高、中、低剂量组,另设10只C57bl/6J小鼠为正常组,连续灌喂给药,观察记录小鼠一般状况,4周后处死小鼠,取血和肾组织,检测血清BUN、Scr值,肾组织HE、PAS、Masson染色,用实时荧光定量PCR法检测肾组织中TIMP-1/MMP-2mRNA的相对表达量。结果:与模型组比较,止消通脉宁高剂量组尿蛋白有下降的趋势,肾功能得到改善,肾脏病理损伤减轻,肾组织TIMP-1/MMP-2mRNA水平均显著降低(P<0.05)。结论:中药复方止消通脉宁能够抑制自发性2型糖尿病KKAy小鼠肾组织TIMP-1/MMP-2mR-NA表达,减轻肾纤维化程度,这可能是其防治DN的作用机制之一。Objective： To observe the influences of zhixiaotongnmining on the kidney MMP - 2 mRNA,TIMP - 1 mRNA expression in kkay mice with diabetic nephropathy. Methods：50 KKAy mice were divided into five groups randomly： model group, valsartan group as the control group, zhixiaotongmaining different dose treatment grous, normal control was 10 C57bl/6J mice. All the mice were sacrificed after treated for 4 weeks, and then used blood serum to test Bun and Scr, The kidney MMP- 2 mRNA,TIMP- 1 mRNA expression was detected by the Real - time fluorescence quantitative polymerase chain reaction. All the data were calculated by SPSS15.0 statistical package. Results ：Mter treatmened by the middle and high dose of zhixiaotongmaining, the MMP -2 mRNA, TIMP - 1 mRNA expression level （ P 〈 0.05 ） in kidney reduced greatly. Conclusion： Zhixiaotongmaining may reduce the MMP - 2 mRNA,TIMP - 1 mRNA expression and to prevent renal fibrosis.

关 键 词：止消通脉宁 糖尿病肾病 KKAY小鼠 TIMP-1 MMP-2 

分 类 号：R285.5[医药卫生—中药学]