Preparative Optimization of Cellulose Microspheres Applied as Supports for High-Performance Liquid Chromatography  

作　　者：陈伟 ZHANG Juan FAN Qingchun BAI Zhengwu 周兴平 XIE Xiaolin 

机构地区：[1]Key Lab for Large-Format Battery Materials and Systems, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology [2]Key Lab of Green Chemical Process, Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2013年第3期460-466,共7页武汉理工大学学报（材料科学英文版）

基　　金：Funded by National Natural Science Foundation of China(Nos.50973086 and 51273073);Hubei Provincial Department of Education of China(No.Z20081501)

摘　　要：The aim of this work is optimizing the techniques to prepare pure cellulose microspheres, which are used as packing adsorbents for high-performance liquid chromatography. Thereupon, cellulose was dissolved in a pre-cooled NaOH/urea solution, from which various-size microspheres were prepared. The volume-average diameters were controlled approximately at 30 p,m, 8 ~tm and 4 pm grades when cyclohexane, liquid paraffin and pump oil were used as dispersants, respectively. The present investigations reveal that higher viscosity dispersant is suitable for the preparation of smaller-size microspheres, while larger size microspheres are prepared preferably using lower-viscosity dispersant. The chiral stationary phase derived from 8 μm grade microspheres can separate the enantiomers of efavirenz.The aim of this work is optimizing the techniques to prepare pure cellulose microspheres, which are used as packing adsorbents for high-performance liquid chromatography. Thereupon, cellulose was dissolved in a pre-cooled NaOH/urea solution, from which various-size microspheres were prepared. The volume-average diameters were controlled approximately at 30 p,m, 8 ~tm and 4 pm grades when cyclohexane, liquid paraffin and pump oil were used as dispersants, respectively. The present investigations reveal that higher viscosity dispersant is suitable for the preparation of smaller-size microspheres, while larger size microspheres are prepared preferably using lower-viscosity dispersant. The chiral stationary phase derived from 8 μm grade microspheres can separate the enantiomers of efavirenz.

关 键 词：CELLULOSE NaOH/urea aqueous solution MICROSPHERE packing material high-performance liquid chromatograph 

分 类 号：TQ352.1[化学工程] TB383.1[一般工业技术—材料科学与工程]