MSG is involved in sperm gelatinolytic activity in the prawn,Macrobrachium rosenbergii  

作　　者：YANG Fan QIAN YeQing MA WenMing LI Ye YANG JinShu YANG WeiJun 

机构地区：[1]Key Laboratory of Conservation Biology for Endangered Wildlife of Ministry of Education and College of Life Sciences,Zhejiang University [2]Ocean Research Center of Zhoushan,Zhejiang University [3]Key Laboratory of Applied Technology of Marine Biology,Ministry of Education,Faculty of Life Science and Biotechnology,Ningbo University

出　　处：《Chinese Science Bulletin》2013年第17期2113-2118,共6页

基　　金：supported by the National Basic Research Program of China (2010CB833803);National Natural Science Foundation of China(40730212,30901093);Zhejiang Marine Biotechnology Innovation Team (2010R50029,2012R10029-14)

摘　　要：Proteases in male reproductive tract are considered to play key roles in fertilization processes.In contrast to mammals,there are limited data concerning crustacean sperm proteases.We previously identified a novel sperm gelatinase(MSG) from Macrobrachium rosenbergii that was inhibited by a male reproduction-related Kazal-type protease inhibitor(MRPINK) specifically.In the present study,MSG was found to be distributed on the vas deferens and terminal ampullae by Western blot.Immunohistochemical analyses revealed that MSG was expressed in secretory epithelial cells and sperm distributed mainly at the base zone.RNA interference(RNAi) mediated knock-down of MSG resulted in a marked loss of sperm gelatinolytic activity.Taken together,the results show that MSG is closely linked to and probably involved in the fertilization process.Proteases in male reproductive tract are considered to play key roles in fertilization processes. In contrast to mammals, there are limited data concerning crustacean sperm proteases. We previously identified a novel sperm gelatinase （MSG） from Macrobrachium rosenbergii that was inhibited by a male reproduction-related Kazal-type protease inhibitor （MRPINK） specifically. In the present study, MSG was found to be distributed on the vas deferens and terminal ampullae by Western blot. Immunohistochemical analyses revealed that MSG was expressed in secretory epithelial cells and sperm distributed mainly at the base zone. RNA interference （RNAi） mediated knock-down of MSG resulted in a marked loss of sperm gelatinolytic activity. Taken together, the results show that MSG is closely linked to and probably involved in the fertilization process.

关 键 词：罗氏沼虾 味精 精子 活性 酶解 蛋白酶抑制剂 对虾 受精过程 

分 类 号：Q55[生物学—生物化学]