Simultaneous polyandry and heteropaternity in tiger(Panthera tigris altaica):Implications for conservation of genetic diversity in captive populations of felids  

作　　者：LIU Dan MA Yue LI HuiYi XU YanChun ZHANG Yan DAHMER Thomas BAI SuYing WANG Jian 

机构地区：[1]Heilongjiang Siberian Tiger Park [2]State Forestry Administration Detecting Center of Wildlife,College of Wildlife Resources,Northeast Forestry University [3]Ecosystems Ltd., [4]Forensic Laboratory of Public Security Department of Heilongjiang Province

出　　处：《Chinese Science Bulletin》2013年第17期2230-2236,共7页

基　　金：supported by the Program for New Century Excellent Talents in University (NCET-10-0280);Technological Development Project of Longyan City (2011LY017)

摘　　要：Male tigers(Panthera tigris altaica) in captivity copulate alternatively with an estrous female,suggesting a potential for heteropaternity as an effective reproductive strategy to maximize genetic diversity of offspring.We analyzed microsatellites to test and compare the genetic output of multiple male mating(simultaneous polyandry) and single male mating(monogamy) with a female in a captive population.Simultaneous polyandry resulted in heteropaternity in 66.7% observed litters.No significant differences between parental populations and between offspring populations were detected in the number of alleles(A),expected heterozygosity(H e),number of effective alleles(N e) per locus and standard individual heterozygosity(SH)(P>0.05 for all 4 indexes).Comparisons showed no significant reduction of A,H o,H e and SH from parental population to offspring population for the two mating modes(P>0.05) except for SH in polyandrous families(P=0.029).However,such reduction was equivalent to single mating families when the influence of relatedness was eliminated using effective SH(E SH)(P>0.05).These results highlight an alternative strategy for managing captive populations of tiger and other wild felids in which animals are combined at one location allowing for copulation by multiple males to encourage heteropaternity in favor of maintained genetic diversity among offspring.Male tigers （Panthera tigris altaica） in captivity copulate alternatively with an estrous female, suggesting a potential for heteropaternity as an effective reproductive strategy to maximize genetic diversity of offspring. We analyzed microsatellites to test and compare the genetic output of multiple male mating （simultaneous polyandry） and single male mating （monogamy） with a female in a captive population. Simultaneous polyandry resulted in heteropaternity in 66.7% observed litters. No significant differences between parental populations and between offspring populations were detected in the number of alleles （A）, expected heterozygosity （He）, number of effective alleles （N_e） per locus and standard individual heterozygosity （SH） （P〉0.05 for all 4 indexes）. Comparisons showed no significant reduction of A, H0, N_e and SH from parental population to offspring population for the two mating modes （P〉0.05） except for SH in polyandrous families （P=0.029）. However, such reduction was equivalent to single mating families when the influence of relatedness was eliminated using effective SH （E_SH） （P〉0.05）. These results highlight an alternative strategy for managing captive populations of tiger and other wild felids in which animals are combined at one location allowing for copulation by multiple males to encourage heteropaternity in favor of maintained genetic diversity among offspring.

关 键 词：遗传多样性保护 圈养种群 猫科动物 东北虎 一夫一妻制 等位基因数 Pgt 杂合度 

分 类 号：Q958[生物学—动物学]