微等离子束对动物皮肤组织的作用效应  

Impact of micro-plasma radio-frequency on animal skin tissue

作　　者：樊昕[1] 石翠萍[1] 韩悦[1] 安俞熙[1] 刘丽红[1] 岳丹霞[1] 杨蓉娅[1] 

机构地区：[1]北京军区总医院全军皮肤损伤修复研究所,100700

出　　处：《中华医学美学美容杂志》2013年第3期164-167,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的观察应用微等离子束对皮肤组织的结构变化及其超微结构变化的作用效应，初步探讨微等离子束的作用机制。方法选择30只豚鼠，随机分为3组，分别给予40W、10kJ，60W、10kJ和80W、10kJ的微等离子束照射，每只豚鼠背部划分为实验侧和空白对照侧2个等分区域，于作用后即刻、1周后和1个月后分别切取实验侧及空白对照侧皮肤行组织病理和透射电镜检测。结果不同剂量设置作用于豚鼠皮肤即刻变化明屁不同．40W、10kJ作用时。表皮基本完整，真皮浅层胶原组织可见轻微均质化；60W、10kJ作用时，表皮局灶性出现点阵状改变，真皮浅层胶原组织出现点阵化和明显均质化；80W、10kJ作用时，表皮完全汽化缺失或者坏死变性，真皮浅中层胶原组织出现大面积均质化。1周后皮肤浅层胶原组织结构逐渐致密，排列有序，1个月后皮肤浅层胶原组织明显增厚，排列致密。透射电镜显示，作用后表皮细胞较完整．细胞间结构正常，但真皮胶原丧失正常结构，细胞结构消失，大量细胞凋亡明显，1个月后仍可见少量细胞凋亡，但胶原结构逐渐恢复。结论微等离子柬对皮肤组织作用有明显的剂量效应，其主要靶组织为真皮胶原组织，可以明显刺激皮肤新生胶原的增生。Objective To investigate the structural and uhrastructural changes of the skin in- duced by micro-plasma radio-frequency technology, and to preliminarily discuss this novel technology mechanism. Methods Thirty guinea pigs were enrolled and randomly divided into three groups. They were radiated by different dose parameters 40 W, 10 kJ; 60 W, 10 kJ and 80 W, 10 kJ. Every guinea pig＇s back was divided into two parts which was removed after immediately, one week and one month, and dermatopathology and transmission electron microscopy （TEM） were performed. Results The different dose setting could make different skin change of immediately effect. When dose setting was 40 W, 10 kJ, skin showed that epidermal cells were integrity and the superficial layer of dermis collagen tissue was light homogenization. When dose setting was 60 W, 10 kJ, epidermal tissue show- ed focal emergence of fractional shape change and obvious homogenization. When dose setting was 80 W, 10 kJ, epidermal showed complete vaporization loss or degeneration necrosis, and dermal su- perficial and middle layer of collagen tissue showed a large area of homogenization. Skin superficial collagen tissue＇s structure gradually showed dense and arranged in an orderly manner after one week and markedly thickened and arranged in compact manner after one month. TEM showed that epider- mal cells were relatively complete, intercellular structure was normal, but the dermal collagen lost o- riginally normal structure and cell structure disappeared and obviously showed massive apoptosis. A small amount of apoptosis was showed but collagen structure gradually restored after one month. Con- clusions The novel micro-plasma radio-frequency has obvious dose effect to skin, and its main target tissues are dermal collagen tissue. It can stimulate skin collagen hyperplasia in certain degree.

关 键 词：微等离子束 皮肤 透射电镜 

分 类 号：R758.733[医药卫生—皮肤病学与性病学]