富血小板纤维蛋白对人脂肪干细胞增殖和成脂分化的影响  

Effect of platelet-rich fibrin on proliferation and adipogenic differentiation of adipose-derived stem cells

作　　者：崔冬[1] 张腾[1] 刁建升[1] 易成刚[1] 郭树忠[1] 

机构地区：[1]第四军医大学西京医院全军整形外科研究所,西安710032

出　　处：《中华医学美学美容杂志》2013年第3期203-206,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的探讨自体富血小板纤维蛋白（platelet-rich fibrin, PRF）对体外培养人脂肪来源干细胞（adipose derived stem ceils, ADSCs）增殖及成脂分化的影响。方法将自愿捐献由脂肪抽吸术获取的脂肪组织进行分离培养ADSCs并鉴定。将第3代ADSCs分为空白对照组和1个PRF膜片组（1PRFM组）和2个PRF膜片组（2PRFM组）。倒置显微镜观察细胞生长情况，培养后1、2、3、4、5、6、7d采用四甲基偶氮噻唑蓝比色法（MTT）法检测细胞增殖活性。在第3、5、7、9、11和14天时采用油红O染色法检测细胞成脂分化情况。结果随着PRFM剂量的增加，细胞增殖数量和成脂率增加，3组差异具有显著统计学意义。结论PRF能明显促进ADSCs增殖和成脂分化，可以作为自体材料应用于脂肪组织工程的研究。Objective To study the effect of autogeneic platelet-rich fibrin （PRF） on prolifera- tion and adipogenic differentiation of human adipose-derived stem cells （ADSCs） in vitro. Methods ADSCs were isolated from adipose tissue obtained from donors undergoing liposuetion and were cul- tured, and underwent identification. ADSCs at passage 3 were divided into three groups： test groups were cultured with 1PRFM and 2PRFM, and control group was cultured without PRF membrane. Tben the growth of the cells was observed by inverted microscope. MTT method was used to observe cell proliferation activity at days 1, 2, 3,4, 5, 6 and 7 after culture. Adipogenic differentiation of ADSCs was observed and quantified by oil red O staining at days 3, 5, 7, 9, 11 and 14. Results Cell proliferation and adipogenie differentiation would be increased with the PRFM, There were signif- icant differenees among three groups. Conclusions PRF could significantly promote proliferation and adipogenic differentiation of ADSCs.

关 键 词：脂肪来源干细胞 富血小板纤维蛋白 细胞增殖 成脂分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]