雌激素对人皮肤成纤维细胞增殖与迁移的影响  

Effect of estrogen on proliferation and migration of human skin fibrnblast

作　　者：左俊[1] 李勤[1] 陈葵[2] 曾东[2] 张斌[2] 余文林[2] 

机构地区：[1]南方医科大学研究生学院,广州510515 [2]广州军区总医院

出　　处：《中华医学美学美容杂志》2013年第3期207-210,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：广东省科技计划项目（雌激素延缓皮肤衰老的分子机制研究,编号：20108031600263）

摘　　要：目的探讨雌激素（17pestrogen，17βE2）对人皮肤成纤维细胞（human skin fibro-blast，HSFB）增殖与迁移的影响。方法分离培养HSFB，传至第4代；（1）四甲基偶氮噻唑蓝比色法（MTT）检测不同浓度17β-E2和17β-E2＋雌激素β受体（estrogen β receptor，ER-β）拮抗剂ICI-182780干预24、48、72、96h后HSFB增殖活力；（2）流式细胞仪检测17β-E2和ICI-182780对HSFB周期分布的影响；（3）建立细胞划痕（Scratch-Wound）模型，24、48、72h后观察HSFB在不同浓度17β-E2和17β-E2＋ICI-182780干预下的迁移情况。结果（1）MTT显示，48、72、96h时，10^-8、10^-9、10^-10、10^-11、10^-12mol／L浓度范围内，均以10μmol／L 17G-E2促增殖效应最强；并且10^-10mol／L17β-E2组（A组）细胞增殖效应强于ICI-182780＋10^-10mol／L 17β-E2组（B组）与空白组（C组，P〈0．01）；（2）A组处于S期的细胞比例多于B、C组（P〈0．01），处于G0／G1期的细胞比例则少于B、C组（P〈0．01）；（3）48h时，10^-6、10^-7、10^-8、10^-9、10^-10mol／L浓度范围内，10mol／L 17β-E2促细胞迁移效应最强（P〈0．01）；24、48、72h时，10μmol／L 178-E2组（D组）细胞迁移距离均大于10^-8mol／L17βE2＋ICI-182780组（E组）及空白组（F组）（P〈0．01）。结论10^-10mol／L浓度雌激素促HSFB增殖效应最强；10μmol／L浓度促HSFB迁移效应最强；ICI-182780有效减弱上述2种效应。Objective To explore the biological effects of estrogen （17β-E2） on the prolifera- tion and migration of human skin fibroblast （HSFB）. Methods HSFBs were isolated and cultured by enzyme digestion. The fourth generation of HSFBs were adopted; （1） the proliferating effect of diverse concentrations of 17β-E2 and 17βE2＋ ICI-182780 on HSFBs was determined with MTT method at 24, 48, 72, 96 h; （2） the influence of 17β E2 and ICI-182780 to HSFBs cycle distribution were deter- mined with flow cytometry; （3） the migration effect of diverse concentrations of 17β-E2 and 17β-E2＋ ICI 182780 on HSFBs was determined at 24, 48, and 72 hours after the creation of the scratch wound in vitro. Results （1） The proliferating speed of HSFBs in 10 ^-10mol/L 17β-E2 group （group A）was the highest of all at 48, 72, 96 h, which was higher than that in ICI-182780＋10^-10mol/L 17β-E2 group （group B） and control group （group C） （P〈0.01） ;（2） the HSFBs during the S phase in group A was more than that in groups B and C （P〈0.01）, while the HSFBs during the G0/G1 phase was less than that in groups B and C （P〈0.01）; （3） the migrating effect of HSFBs in 10^-8mol/L 17β-E2 group （group D） was the highest of all at 48 h, which was higher than that in ICI-182780＋10^-10mol/L con trol group （group E）and control group （group F） （P〈0. 01）. Conclusions The concentration of 10^-10mol/L estrogen has the strongest effect of promoting proliferation and that of 10 8 mol/L has the strongest chemotaxis; ICI-182780 can abate the above effect effectively.

关 键 词：人皮肤成纤维细胞 雌激素 细胞增殖 细胞迁移 ICI-182780 

分 类 号：R285[医药卫生—中药学]