氨氯地平经皮肤渗透给药对缺血随意型皮瓣成活的影响  

Effects of amlodipine through percutaneous penetration on survival of ischemic random skin flap

作　　者：秦永红[1] 张选奋[1] 李爱书 钟琳[1] 刘凯[1] 张瑾[1] 

机构地区：[1]兰州大学第二医院整形外科,730030 [2]甘肃省定西市安定区妇幼保健站

出　　处：《中华医学美学美容杂志》2013年第3期211-214,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：兰州大学医学科研基金资助项目（编号：LZUYX200822）

摘　　要：目的探讨氨氯地平水凝胶（amlodipinegel）经皮肤渗透给药及其对大鼠缺血随意型皮瓣成活的影响。方法制备0．5％、1．0％、1．5％、2．0％和2．5％氨氯地平水凝胶。用改良的Franz扩散池测定氨氯地平经大鼠皮肤的累积渗透量。在大鼠缺血随意型皮瓣外用氨氯地平水凝胶7d后，测定皮瓣的成活面积和给药后第2、6小时皮瓣组织中氨氯地平的含量。结果氨氯地平的累积渗透量随氨氯地平浓度和渗透时间增加而增加（P〈0.05）。0．5％、1．0％氨氯地平水凝胶中的氨氯地平的累积渗透量〈1．5％、2．0％、2．5％氨氯地平水凝胶（P〈0．05）。1．5％氨氯地平水凝胶组皮瓣组织内氨氯地平的含量均〉0．5％氨氯地平水凝胶组（P〈0．05）。0．5％氨氯地平水凝胶组皮瓣的成活面积为（391．4±65．4）mm^2，高于凝胶基质对照组的（192．9±56．8）mm^2及空白对照组的（191．0±50．2）mm^2（P〈0．05），但1．5％氨氯地平水凝胶组皮瓣（265．7±88．3）mm^2与两对照组问差异无显著统计学意义（P〉0．05）。结论氨氯地平可渗透进入皮肤组织；0．5％氨氯地平水凝胶可显著提高缺血随意型皮瓣的成活面积。Objective To investigate the penetrative ability of amlodipine gels and to evaluate their effect on the survival of rat dorsal ischemic random skin flaps. Methods 0. 5%, 1. 0%, 1.5%, 2.0%, and 2.5% amlodipine gels were made. The accumulative penetrative quantities of amlodipine through rat skin were assayed with a modified Franz＇s diffusion cell in vitro. Pure gel with- out amlodipine, 0.5% and 1.5 9%amlodipine gel were respectively applied on rat random ischemie skin flap once a day for 7 days. The viable area was measured on the seventh postoperative clay and the quantities of amlodipine within skin flap were also detected at 2 and 6 hours after application of amlo- dipine gel. Results The accumulative penetrative quantities of amlodipine increased in time-and con- eentration-dependent manner （P〈0.05）. Accumulative quantities of 0.5% and 1.0% amlodipine gel were lower than those of 1. 5 %, 2.0%, and 2.5% gel, respectively （P〈0.05）. The quantities of amlodipine within flap tissue in 1.5 % amlodipine gel was higher than that of 0.5% amlodipine gel （P〈0.05）. The survival area of ftap in 0.5% amlodipine gel group （391.4±65.4） mm^2 was higher than those of the pure percutaneous gel group （192.9±56.8） mm^2 and the control group （191.0± 50.2） mm2 （P〈0.05）, but no significant difference was seen between 1. 5 % amlodipine gel group （265.7±88.3） ram2 and control group （P〉0.05）. Conclusions Amlodipine could penetrate into skin tissues. 0.5% amlodipine gel could significantly increase survival area of ischemic random skin flap.

关 键 词：氨氯地平 皮肤渗透给药 随意型皮瓣 坏死 

分 类 号：R9[医药卫生—药学]