褪黑素及受体表达对性发育启动的调节作用  

Regulation effect of expressions of melatonin and its receptors on puberty development

作　　者：周巧利[1] 石星[1] 倪世宁[1] 顾威[1] 朱子阳[1] 王旭[1] 刘倩琦[1] 

机构地区：[1]南京医科大学附属南京儿童医院内分泌病科,江苏省210008

出　　处：《江苏医药》2013年第11期1247-1249,共3页Jiangsu Medical Journal

基　　金：南京市卫生局科技发展青年基金(QYK09161)

摘　　要：目的探讨褪黑素(MT)及MT受体(MT1、MT2)在性发育启动调节中的作用。方法40只SD雌鼠按不同发育阶段均分为幼年组(A组)、青春前期组(B组)、青春早期组(C组)和性发育成熟期组(D组),分别于生后第15、25、35、45天处死。采用酶联免疫法检测各组血浆MT、促黄体生成素(LH)水平,荧光实时定量-PCR(RT-PRC)检测各组下丘脑组织中促性腺激素释放激素(GnRH)以及MT1、MT2mRNA表达水平。结果血浆MT水平随大鼠发育程度而下降(P<0.05);C组血浆LH水平显著高于A、B组(P<0.05)。A组GnRH mRNA表达水平最低,C组最高(P<0.05);A、B组MT1mRNA表达水平较高,性发育启动后显著下降(P<0.05);四组MT2mRNA表达水平相仿。结论 MT及其受体在青春发育中起抑制作用,参与GnRH的调控,与青春发育启动有关。Objective To explore the regulating effect of expressions of melatonin（MT） and its receptors（MT1,MT2） on puberty development. Methods On the basis of developmental phrases,40 SD female rats were equally divided into four groups of A （juvenile）, 13 （preaclolescence）, C （early adolescence） and D（maturity）,which were executed on the 15th ,25th, 35th and 45th day after birth, respectively. Plasma levels of MT and luteotropic hormone （LH） were detected by ELISA, and gonadotrophin releasing hormone（GnRH） mRNA, MT1 mRNA and MT2 mRNA were detected by RT-PCIL Results Plasma level of NiT decreased during puberty development（P〈0. 05）. Plasma level LH was higher in group C than that in groups of A and B（P〈0. 05）. Plasma level of GnRH mRNA was the highest in group C and the lowest in group A（P〈0. 05）. Expression of MT1 mRNA was significantly higher in groups of A and B, which was reduced in groups of C and D（P〈0. 05）. Conclusion Plasma MT and its receptors have inhibitory effect on puberty development, which are involved in the regulation of GnRH and related to evocation of puberty development.

关 键 词：褪黑素 褪黑素受体 性发育 

分 类 号：R335[医药卫生—人体生理学]