基于微卫星标记整合长牡蛎遗传图谱  

Microsatellite-based consensus map of the Pacific oyster(Crassostrea gigas)

作　　者：郭香[1] 李琪[1] 孔令锋[1] 于红[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《水产学报》2013年第6期823-829,共7页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2012AA10A405-6);国家重点基础研究发展计划(2010CB126406)

摘　　要：为了提高长牡蛎遗传图谱上的微卫星标记密度,实验采用6个家系图谱间的共有微卫星标记作为锚定标记,构建了长牡蛎的整合图谱。该整合图谱共有161个微卫星标记,覆盖10个连锁群,图谱长度和平均间距分别为615.4和3.8 cM。各连锁群的标记数介于10～24个之间,连锁群长度为47.3～73.3 cM,是目前密度最高的长牡蛎微卫星图谱。不同作图家系连锁群上的标记分组保持一致,但标记顺序出现差异,可能与长牡蛎自然群体中存在大量的染色体重排现象有关。结果表明,该图谱可以为今后长牡蛎的遗传育种研究提供新的遗传工具。In order to improve the density of microsatellite markers, a consensus map of C. gigas was constructed based on common microsatellite markers among six mapping populations. The consensus map contained 161 microsatellite markers, spanning 10 linkage groups. The map length and average genetic distance is 615.4 cM and 3.8 cM respectively. The number of loci on linkage group ranged from 10 to 24 and the genetic length of linkage group varied from 47.3 to 73.3 cM. At present,this consensus map is the densest SSR-based map of C. gigas. Among different mapping populations, the grouping of markers is consistent;however,the order of markers is a little different, which is likely attributed to polymorphism for chromosomal rearrangements in the natural population of Pacific oysters. The results show that the consensus map will provide a new reference tool for genetics and breeding of C. gigas in the future.

关 键 词：长牡蛎 微卫星 整合图谱 染色体重排 共显性标记 

分 类 号：Q785[生物学—分子生物学]