Differential Regulatory Mechanisms of CBF Regulon Between Nipponbare (Japonica) and 93-11 (Indica) During Cold Acclimation  

作　　者：PAN Xiao-wu LI Yong-chao LI Xiao-xiang LIU Wen-qiang MING Jun LU Ting-ting TAN Jiang SHENG Xin-nian 

机构地区：[1]Rice Research Institute,Hunan Academy of Agriculture Science

出　　处：《Rice science》2013年第3期165-172,共8页水稻科学（英文版）

基　　金：supported by the Key Project of the National Twelve-Five Year Research Program of China(Grant No.2012BAD20B05-01-1);the Projects from the National Rice Industrial Technology System(Grant No.nycytx-001);the Grant Special Foundation of Transgenic Plants in China(Grant No.2009ZX08001-009B)

摘　　要：Nine CBF/DREB1 homologous genes in rice were obtained by BLAST search in the NCBI database, which share conserved amino acid sequences with DREB1 protein in Arabidopsis. Three CBF genes organized in tandem, named OsCBF1, OsCBF2 and OsCBF3, showed a transient induction in the process of cold acclimation, much stronger in indica rice 93-11 compared with japonica rice Nipponbare. The candidate downstream genes OsLIP5 and OsLIP9 were induced in 93-11 but not in Nipponbare. The differential expression of CBF regulon might be caused by polymorphisms within promoter sequences between these two rice varieties. These results could be useful for utilization of CBF/DREB1 genes and illustration of differences in chilling tolerance between indica and japonica rice varieties.Nine CBF/DREB1 homologous genes in rice were obtained by BLAST search in the NCBI database, which share conserved amino acid sequences with DREB1 protein in Arabidopsis. Three CBF genes organized in tandem, named OsCBF1, OsCBF2 and OsCBF3, showed a transient induction in the process of cold acclimation, much stronger in indica rice 93-11 compared with japonica rice Nipponbare. The candidate downstream genes OsLIP5 and OsLIP9 were induced in 93-11 but not in Nipponbare. The differential expression of CBF regulon might be caused by polymorphisms within promoter sequences between these two rice varieties. These results could be useful for utilization of CBF/DREB1 genes and illustration of differences in chilling tolerance between indica and japonica rice varieties.

关 键 词：RICE CBF/DREB1 cold acclimation gene expression promoter analysis 

分 类 号：S511[农业科学—作物学]