MMP-9-1562位点T等位基因与儿童EV71重症脑炎易感性关系的研究(英文)  

Association of Functional-1562 C/T Polymorphism in Matrix Metalloproteinase-9(MMP-9) with Risk of Severe Encephalitis in Enterovirus 71 Infected Children

作　　者：辛丹丹[1] 陈宗波[1] 刘相萍[1] 韩振靓[1] 李继安[1] 刘培培[1] 胡静飞[1] 韩伟平[1] 

机构地区：[1]青岛大学医学院附属医院儿科,山东青岛266003

出　　处：《现代生物医学进展》2013年第15期2870-2874,共5页Progress in Modern Biomedicine

基　　金：National Science Foundation of China(31171212)~~

摘　　要：目的：研究基因MMP-9-1562位点基因多态性与肠道病毒71型（EV71）脑炎的关系,探讨不同基因型对EV71脑炎患病风险及病情程度的影响。方法：运用限制性片段长度多态性-聚合酶链反应（PCR-RFLP）检测EV71感染阳性病毒性脑炎患儿及正常对照组儿童基因组MMP-9-1562位点碱基变异情况。结果：EV71脑炎重症组MMP-9-1562位点T等位基因频率分布明显高于EV71脑炎轻症组（16%VS 7.5%,OR 2.362,95%CI 1.170~4.768,P〈0.05）。MMP-9-1562位点T等位基因携带者感染EV71后可明显增加神经系统并发症的发生率。EV71脑炎患儿MMP-9-1562位点T等位基因频率分布与正常对照儿童比较无明显差异。结论：MMP-9-1562位点T等位基因携带者在感染EV71后易发展为重症中枢神经系统感染;MMP-9-1562位点T等位基因可增加EV71感染后神经系统并发症的发生率;MMP-9-1562位点单核苷酸多态性与EV71脑炎的发病率无关。Objective： To investigate the relationship between the polymorphisms of matrix metalloproteinase-9（MMP-9）-1562 and the susceptibility of enterovirus71 encephalitis,and explore the risk factors of EV71 encephalitis.Methods： Enterovirus 71 in the feces and cerebrospinal fluid of encephalitis children was detected by reverse transcription polymerase chain reaction（RT-PCR）,and EV71 specific primer was used PCR-restriction fragment length polymorphism（PCR-RFLP） techniques were performed to determine the genotypes of MMP-9 gene in encephalitis group and in control group.Results： The MMP-9-1562 T allele was observed with sigificantly greater frequency in patients with serious EV71 encephalitis compared with slight EV71 encephalitis patients（16 % VS 7.5 %,OR 2.362,95 % CI 1.170~4.768,P0.05）.T alleles carriers of MMP-9-1562 can increase the incidence of nervous complications after infection EV71.However,for the allele distribution of the MMP-9-1562C/T polymorphism,there was no significant difference between EV71 encephalitis patients and healthy children.Conclusion： MMP-9-1562 T allele carriers in EV71 infection may develop into severe central nervous system（CNS） infection.T alleles of MMP-9-1562 can increase the incidence of nervous complications after EV71 infection,but the MMP-9-1562C/T polymorphism is not associated with risks of either EV71 encephalitis or healthy children.

关 键 词：肠道病毒71型(EV71) 脑炎 金属蛋白酶-9(MMP-9) 基因多态性 

分 类 号：Q75[生物学—分子生物学] R512-3[医药卫生—内科学]