盐酸小檗碱对胰岛β细胞内质网应激相关凋亡的影响  

Effect of berberine on the endoplasmic reticulum stress-related apoptosis in pancreatic β cells

作　　者：邬姗[1] 陆付耳[1] 董慧[1] 徐丽君[1] 杨明炜[1] 王开富[1] 邹欣[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030

出　　处：《中国糖尿病杂志》2013年第6期544-547,共4页Chinese Journal of Diabetes

基　　金：国家自然科学基金资助项目(30772853)

摘　　要：目的探讨盐酸小檗碱(BER)对胰岛β细胞内质网应激相关凋亡的影响。方法以衣霉素(Tun)诱导胰岛β细胞株MIN6细胞内质网应激,检测BER对细胞增殖、凋亡和胰岛素分泌,及对细胞凋亡相关蛋白ASK1、Caspase-12表达的影响。结果 BER(1、10μmol/L)对内质网应激细胞基础和葡萄糖刺激的胰岛素分泌均无明显影响,可减少细胞凋亡、抑制凋亡相关蛋白ASK1和Caspase-12的表达。结论 BER能减少胰岛β细胞内质网应激相关凋亡,其机制可能与抑制凋亡相关蛋白ASK1和Caspase-12的表达有关。Objective To explore the effect of berberine on the endoplasmic retieulum stress-related apoptosis in pancreatic β cells. Methods The endoplasmic retieulum stress in MIN6 cells was induced by Tunicamycin. The effect of berberine on cell proliferation and apoptosis, insulin secretion, expression of apoptosis-related proteins ASK1 and Caspase-12 were examined. Results Basal and glucose-stimulated insulin secretion were not affected while cell apoptosis was decreased by berberine （1, 10μmol/L）, however, the expression of ASK1 and caspase-12 was inhibited significantly by berberine. Conclusion Berberine can reduce the endoplasmic reticulum stress-related apoptosis in pancreatic β cells. The mechanism may be related to the inhibition of ASK1 and easpase-12 expressions.

关 键 词：小檗碱 内质网应激 凋亡 胰岛Β细胞 

分 类 号：R587.1[医药卫生—内分泌]