乙型肝炎病毒感染人群T细胞对其抗原蛋白免疫应答的特征分析  

Characteristics of T cell′s immune response to hepatitis B virus antigenspecificity protein in population with different types of the infections

作　　者：廖崇伦 陈萍[2] 

机构地区：[1]武穴市第一人民医院检验科,湖北武穴435400 [2]郧阳医学院附属人民医院内科,湖北十堰442000

出　　处：《中华医院感染学杂志》2013年第12期2791-2792,2795,共3页Chinese Journal of Nosocomiology

基　　金：湖北省卫生厅基金项目(1000970976)

摘　　要：目的研究不同类型的乙型肝炎病毒(HBV)感染者的HBV抗原特异性T细胞免疫应答水平,为临床免疫调节治疗HBV感染提供诊断依据。方法回顾性分析2010年1月-2011年1月医院就诊并且符合纳入标准的受试者80例,按照慢性乙型肝炎类型分为3组,分别是肝炎肝硬化(LC)组、无症状慢性HBV携带者(ASC)组、慢性乙型病毒性肝炎(CHB)组,所有患者均采取酶联接免疫吸附剂测定的方法(ELISA法)测定T细胞因子的免疫应答水平。结果 LC组、ASC组、CHB组的一般资料,组间比较差异无统计学意义,具有可比性;LC组和CHB组的INF-r细胞因子表达水平(1.21±0.16)、(1.57±0.21)明显高于ACS组(1.13±0.12),在INF-r、TGF-β-1、IL-17、IL-10 4个细胞因子代表的T细胞亚组CHB平均水平最高,分别为(1.57±0.21)、(3.55±0.25)、(1.36±0.12)、(32.66±11.22),差异有统计学意义(P<0.05)。结论不同HBV感染者的抗原特异性T细胞的免疫应答不同,在一定程度上,对临床免疫调节治疗有指导意义。OBJECTIVE To study the T cellrs immune response to HBV antigen-specificity protein in the patients with different types of hepatitis B virus （HBV） infections so as to guide the treatment of HBV infections with immunomodulatory. METHODS A total of 80 patients who met the standard and have been treated at the hospital from Jan 2010 to Jan 2011 were selected as the study objects, the clinical data were retrospectively analyzed. The patients were divided into three groups according to the types of chronic hepatitis B, namely the hepatitis with hepatic cirrhosis （LC） group, the chronic hepatitis b virus carrier without symptoms （ASC） group, and the chronic hepatitis B （CHB） group. The levels of T cell＇s immune response of all the patients were determined by using enzyme-linked immunosorbent assay method. RESULTS The difference in the general data between the LC group, ASC group, and the CHB group was not statistically significant, showing comparability. The expression level of IFN-γ was （1.21±0. 16）in the LC group, （1. 57±0. 21）in the CHB group, significantly higher than （1.13 ± 0. 12） of the ACS group. The levels of INF-γ, TGF-β-1, IL-17, and IL-10L-17 that represented the subtypes of the T cell were the highest, which were（1.57±0.21）, （3.55±0.25）, （1.36!0.12）, and（32.66± 11.22）, respectively, the difference was statistically significant. CONCLUSION The patients with different types of HBV infections vary in the T cellrs immune response to the antigen-specificity protein, which can, to a certain extent, guide the clinical treatment with immunomodulatory.

关 键 词：乙型肝炎病毒 T细胞 免疫应答 方差分析 

分 类 号：R512.62[医药卫生—内科学]