星点设计-效应面法优选岗松总黄酮提取工艺  

Optimization of Extraction Technology for Total Flavonoids from Baeckea frutescens L. by Central Composite Design Response Surface Methodology

作　　者：邱宏聪 刘布鸣 

机构地区：[1]广西壮族自治区中医药研究院,南宁530022 [2]广西中药质量标准研究重点实验室,南宁530022

出　　处：《中国实验方剂学杂志》2013年第12期46-49,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西壮族自治区卫生厅中医药科技专项(GZPT1229);广西中药质量标准重点实验室主任基金项目(桂中重科01105)

摘　　要：目的:优选岗松总黄酮的提取工艺条件。方法:以总黄酮含量为指标,乙醇体积分数、固液比、提取时间为考察因素,采用星点设计-效应面法优选岗松中总黄酮的提取工艺,并进行预测分析。结果:最佳提取工艺为加16倍量70%乙醇提取3次,每次60 min,总黄酮平均质量分数1.43%。结论:实测值与预测值吻合度高,预测性良好,优选的提取工艺合理可行。Objective： To optimize extraction technology of total flavonoids from Baeckea frutescens L.Method： With the content of total flavonoids as index,ethanol concentration,solid-liquid ratio and extraction time as factors,central composite design and response surface methodology was used for optimize of extraction technology of total flavonoids from B.frutescens L.,then predicted optimal exaction process.Result： Optimum extraction technology conditions were as follows： extracted 3 times with 16 times the amount of 70% ethanol,60 min each time.Under this technology,the average mass fraction of total flavonoids was 1.43%.Conclusion： The observed value and the predicted value were close,which proved that optimizied technology was reasonable and feasible with good predictability.

关 键 词：星点设计 效应面法 岗松 提取工艺 

分 类 号：R283.6[医药卫生—中药学]