高压消解-原子荧光光谱法测定天麻药材及全天麻胶囊中硒的含量  

Determination of Selenium in Gastrodia elata and Quantianma Capsule by Atomic Fluorescence Spectrometry with High Pressure Digestion

作　　者：陈思颖[1] 兰波[1] 朱迪[1] 刘童[1] 王爱民[1] 王永林[1] 

机构地区：[1]贵阳医学院药学院,贵阳550004

出　　处：《中国实验方剂学杂志》2013年第12期79-82,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI13B04);贵州省重大专项(黔科合重大专项字[2011]6005);贵阳市重大专项(筑科合同[2011401]社6-1号);科技部国际合作重大项目(2010DBF30530)

摘　　要：目的:建立高压消解-原子荧光光谱测定天麻药材及全天麻胶囊中硒的含量测定方法。方法:采用高压消解法处理天麻药材及全天麻胶囊样品,用具有还原与消除干扰作用的硝酸-盐酸混合体系作为反应介质,并加入铁氰化钾消除共存离子的干扰,用氢化物发生-原子荧光分光光度法对天麻药材及全天麻胶囊中硒含量测定进行研究,同时以茶叶、黄芪、人参成分分析标准物质对测定结果进行质量控制。结果:硒的荧光强度与进样浓度之间呈良好的线性关系,检出限为0.16μg.L-1,重复性、精密度均良好,回收率98.26%～101.2%,RSD 2.4%～4.4%,采用所建立的方法对22批不同产地天麻药材和5批全天麻胶囊中硒含量进行了测定,结果天麻药材中硒的含量为0.012 4～0.660 mg.kg-1,表明各产地由于地理环境不同,差异较大;全天麻胶囊由于天麻药材来源较为一致,硒含量为0.214～0.385 mg.kg-1。结论:方法操作简便、成本低廉,且对环境污染小,适用于天麻中硒的含量测定,为进一步提高天麻药材及全天麻胶囊质量控制及其再开发应用提供实验依据。Objective： The research aimed to establish a high pressure digestion and a hydride-generation atomic fluorescence spectrometry（HG-AFS） method for the determination of selenium in Gastrodia elata and Quantianma capsules.Method： The samples,include G.elata herbs,Quantianma capsules and CRM Tea,Astragalus and Ginseng were prepared by high pressure digestion.The reaction medium was HNO3-HCl,which had reducing and interference diminishing effects.K3Fe（CN） 6 was added to diminish the interference of coexist elements.Selenium in G.elata and Quantianma capsules is researched by HG-AFS.Meanwhile,selenium in the CRM Tea,Astragalus and Ginseng was detected in order to control the quality of the measured results.Result： There was a good linear relation between fluorescence intensity and concentration.The LOD was 0.16 μg.L-1.The precision and repeatability were satisfactory.The recovery was 98.26%-101.2% and RSD was 2.4%-4.4%.The method was used to detect the concentration of selenium in 22 batches of G.elata herbs and 5 batches of Quantianma capsules.The concentration of selenium in 22 batches of herbs is 0.012 4-0.660 mg.kg-1.The content was obviously different due to the different origins.As the origin of the Quantianma capsules is same,the concentration of selenium is 0.214-0.385 mg.kg-1.Conclusion： The method is easy and simple to handle.It has low cost and little pollution to environment.This method can be applied into the determination of selenium in G.elata and Quantianma capsules.It provides useful references for the quality control and further application of G.elata and Quantianma capsules.

关 键 词：高压消解 原子荧光光谱法 天麻 全天麻胶囊 硒 

分 类 号：R284.1[医药卫生—中药学]