不同产地粉萆薢总皂苷含量测定  

Method for Determination of Total Saponins in Dioscoreae hypoglauca from Different Areas

作　　者：李淑青[1] 肖潇[1] 王坤凤[1] 吴玲芳[1] 张兰珍[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2013年第12期118-120,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京中医药大学科研创新团队项目(2011-CXTD-12)

摘　　要：目的:建立粉萆薢总皂苷含量测定方法。方法:以薯蓣皂苷元为对照品,高氯酸为显色剂,测定波长408 nm,采用可见分光光度法测定了5个不同产地粉萆薢药材总皂苷含量。结果:薯蓣皂苷元线性范围为1.55～12.42 mg.L-1,r=0.999 5(n=6),加样回收率为100.69%,RSD 2.75%,不同产地粉萆薢总皂苷含量分别为广西玉林2.85%,RSD 1.32%;广西南宁2.76%,RSD 1.16%;浙江临安2.54%,RSD 1.38%;湖北宜昌1.94%,RSD 1.83%;四川崇州0.84%,RSD 1.75%。结论:测定方法简便,准确,重复性好,可作为粉萆薢总皂苷的含量测定。Objective： The method for determination of total saponins in Dioscoreae hypoglauca from different areas was developed.Method： Spectrophotometer was used to determine the total saponins with perchloric acid for colorimetric reaction and diosgenin as the reference substance at 408 nm as measurement wavelength.Result： The linearity range of diosgenin was 1.55-12.42 mg.L-1（r = 0.999 5）.The average recovery was 100.69%,RSD 2.75%.The content of total saponins in D.hypoglauca from different areas was 2.85%（Yulin,Guangxi）,2.76%（Nanning,Guangxi）,2.54%（Linan,Zhejiang）,1.94%（Yinchang,Hubei）,0.84%（Chongzhou,Sichuan） respectively.Conclusion： The method was simple,accurate and reproducible,which could be applied to the determination of saponins in D.hypoglauca from different areas.

关 键 词：粉萆薢 总皂苷 不同产地 

分 类 号：R284.1[医药卫生—中药学]