HPLC测定不同采收期香青藤中肉桂酸苯乙酯含量  

HPLC Determine Contents of β-phenylethyl Cinnamate in Illigera aromatic from Different Collection Periods

作　　者：谢丽莎[1] 龚志强[1] 李娉[2] 杨力龙 

机构地区：[1]广西中医药大学赛恩斯新医药学院,南宁530222 [2]广东省妇幼保健院,广州510010

出　　处：《中国实验方剂学杂志》2013年第12期136-138,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西壮族自治区科技攻关项目(桂科攻0815005-1-12)

摘　　要：目的:考察香青藤不同采收期肉桂酸苯乙酯的含量,探索药材最佳采收期,为控制香青藤药材质量提供依据。方法:采用高效液相色谱法(HPLC)测定肉桂酸苯乙酯,Ultimate C18(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(37∶63),流速1.0 mL.min-1,检测波长280 nm,柱温30℃。结果:香青藤药材中肉桂酸苯乙酯含量随采收期不同而差别而各异,其中以8,9月份采收含量较高。结论:所建立的方法简便、准确、重复性好,为香青藤质量控制及规范化种植提供理论基础。Objective： To determine of the contents of β-phenylethyl cinnamate in Illigera aromatic from different collection periods,to discover the inherent rule about the content change of different collection periods.Method： Using Ultimate C18（4.6 mm ×250 mm,5 μm） 0.1% the phosphate buffer solution-acetonitrile（37∶63） as mobile phase,the column temperature was at 30 ℃,the flow rate 1.0 mL.min-1and detection wavelength was 280 nm.Result： The content of β-phenylethyl cinnamate varied in different collection periods,with the highest point in August and January.Conclusion： This method has good sensitivity,precision and simplicity,offers the basis of controlling the quality of I.aromatic and the realization of good agricultural practice.

关 键 词：高效液相色谱法 香青藤 肉桂酸苯乙酯 

分 类 号：R284.1[医药卫生—中药学]