丹参总酚酸在大鼠血浆中的药动学研究  

Pharmacokinetic Study of Total Phenolic Acids of Salvia miltiorrhiza in the Rat Plasma

作　　者：王小平[1,2] 刘峰[2] 杨东花[1] 马存德[2] 

机构地区：[1]陕西中医学院,陕西咸阳712046 [2]陕西步长制药有限公司,陕西咸阳712000

出　　处：《中国实验方剂学杂志》2013年第12期141-144,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省中医药管理局项目(ZY07);咸阳市科学技术局项目[2011K13-01(6)]

摘　　要：目的:考察丹参总酚酸中主要成分紫草酸、丹酚酸B在大鼠体内的药代动力学过程。方法:采用血药浓度法,HPLC法测定大鼠ig(1.0 g.kg-1)丹参总酚酸后不同时间点的血药浓度,DAS3.0软件计算药动学参数。结果:紫草酸在1.0～20.0 mg.L-1线性关系良好(R2=0.993 1);回收率在97.15%～100.21%,日内、日间RSD<10.23%;丹酚酸B在2.0～20.0mg.L-1线性关系良好(R2=0.992 5);回收率在94.80%～99.96%,日内、日间RSD<10.06%。紫草酸的t1/2为19.4 min,Cmax为14.9 mg.L-1,AUC为418.0.μg.L-1.min-1。丹酚酸B的t1/2为27.2 min,Cmax为17.1 mg.L-1,AUC为831.3μg.L-1.min-1。结论:HPLC选择性好,灵敏度高,适用于血浆样品成分含量的测定,为进一步的复方药代动力学的研究奠定了基础。Objective： To investigate pharmacokinetics process of the major components of the total phenolic acids of Salvia miltiorrhiza in rats.Method： Using HPLC to measure plasma concentration at different time after ig total phenolic acids of S.miltiorrhiza was determined;DAS 3.0 software was used to calculate pharmacokinetic parameters.Result： Lithospermic acid was linear in the range of 1.0-20.0 mg.L-1.Absolute recoveries were between 97.15%-100.21%.The RSD of intra-day and inter-day was less than 10.23%.Salvianolic acid B was linear in the range of 2.0-20.0 mg.L-1.Absolute recoveries were between 94.80% 99.96%.The RSD of intra-day and inter-day was less than 10.06%.Pharmacokinetic parameters of lithospermic acid were as follows： t1 /2 = 19.4 min,Cmax = 14.9 mg.L-1,AUC = 418.0 μg.L-1.min-1.Pharmacokinetic parameters of salvianolic acid B were as follows： t1 /2 = 27.2 min,Cmax = 17.1 mg.L-1,AUC =831.3 μg.L-1.min-1.Conclusion： HPLC had good selectivity,high sensitivity for the determination of plasma sample.for the pharmacokinetic studies of lithospermic acid and salvianolic acid B in total phenolic acids of S.miltiorrhiza

关 键 词：丹参总酚酸 紫草酸 丹酚酸B 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]