参七消痞颗粒对慢性萎缩性胃炎大鼠血清GH,EGF,GAS的影响  

Effect of Senqi Xiaobi Granules on Serum Levels of GH,EGF and GAS in Rats with Chronic Atrophic Gastritis

作　　者：李俊青[1] 李纯[1] 刘希[1] 王晶[1] 孙建宁[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中国实验方剂学杂志》2013年第12期172-175,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项重大新药创制(2010ZX09102-211)

摘　　要：目的:观察参七消痞颗粒(SQ)对慢性萎缩性胃炎大鼠血清生长激素(growth hormone,GH)、表皮生长因子(epidermal growth factor,EGF)、胃泌素(gastrin,GAS)的影响。方法:通过N-甲基-N'-硝基-N-亚硝基胍(MNNG)负荷其他因素建立慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠模型(14周),之后再随机分为模型组、摩罗丹组、叶酸组、参七消痞颗粒2,4,8 g.kg-1组。模型组给予生理盐水10 mL.kg-1,摩罗丹组灌胃摩罗丹3.6 g.kg-1,叶酸组灌胃叶酸2 mg.kg-1,共计8周。另从实验开始造模时就设立正常对照组。每周监测大鼠体重,第22周取材,观察大鼠胃的病理组织学改变,腹主动脉取血分离血清,放免法检测血清GH,EGF,GAS的含量。结果:从第3周开始模型组大鼠与正常对照组相比,体重明显较轻(P<0.05);第22周,模型组大鼠胃容物恶臭,胃黏膜苍白,胃壁较薄,胃黏膜皱襞走向紊乱,HE切片镜下观察,固有层腺体减少,排列不规则,间隙较大,肠上皮化生明显,有异型增生,参七消痞颗粒各剂量组与模型组相比,肉眼及镜下观察均有不同程度的改善作用;血清GH水平,参七消痞颗粒8 g.kg-1组与模型组比较显著升高[(5.42±0.68),(5.02±0.71)μg.L-1,P<0.05)];血清EGF水平,参七消痞颗粒4,2 g.kg-1组与模型组比较显著升高[(0.88±0.22),(0.86±0.18),(0.72±0.17)μg.L-1,P<0.01)];血清GAS水平,参七消痞颗粒4,2 g.kg-1组与模型组比较显著降低[(68.07±11.38),(62.42±10.19),(74.85±7.06)ng.L-1,P<0.05或P<0.01)]。结论:参七消痞颗粒能明显改善慢性萎缩性胃炎大鼠的胃黏膜病变,其治疗机制可能与升高血清EGF水平、降低血清GAS水平有关。Objective： To evaluate the effect of Senqi Xiaobi（SQ） granules on serum levels of growth hormone（GH）,epidermal growth factor（EGF） and gastrin（GAS） in rats with chronic atrophic gastritis（CAG）.Method： CAG model was established by N-methyl-N’-nitro-N-nitrosoguanidine（MNNG） and other comprehensive factors for 14 weeks.Then the model rats were divided into 6 groups randomly,which were ig administered with saline,Moluodan,folic acid,SQ granules 2,4,8 g.kg-1,respectively,taking normal Wistar rats as control group at the beginning of modeling.Body weight of rats was observed for 22 weeks.In 22nd week,the pathological changes of gastric mucosa were observed,serum levels of GH,EGF GAS were measured by radioimmunoassay.Result： in 22 nd week,in the model group,there were typical CAG pathological morphological changes on the gastric mucosa,Compared with model group,there were improvements on pathological changes at different degrees in the SQ Granules-treated groups;serum levels of GH in the SQ Granules 8 g.kg-1 group were significantly higher than that in the model group [（5.42 ± 0.68）,（5.02 ± 0.71 μg.L-1,P ＆lt;0.05） ];serum levels of EGF in the SQ granules 4,2 g.kg-1 group were significantly higher than that in the model group [（0.88 ±0.22）,（0.86 ± 0.18）,（0.72 ± 0.17） μg.L-1,P ＆lt; 0.01） ];serum levels of GAS in the SQ granules 4 g.kg-1 group and SQ granules 2 g.kg-1 group were significantly lower than that in the model group [（68.07 ±11.38）,（62.42 ±10.19）,（74.85 ±7.06） ng.L-1,P ＆lt;0.05 or P ＆lt;0.01） ].Conclusion： SQ granules improve mucosal atrophy possibly by increasing serum levels of GH,EGF and reducing serum levels of GAS in rats with chronic atrophic gastritis.

关 键 词：慢性萎缩性胃炎 参七消痞颗粒 生长激素 表皮生长因子 胃泌素 

分 类 号：R285.5[医药卫生—中药学]