补肾、健脾和补肾健脾方对尾部悬吊大鼠钙、磷代谢的影响  

Effects of Tonifying Kidney and Invigorating Spleen Prescription on the Metabolism of Calcium and Phosphorus in Rat Tail Suspension Model

作　　者：张林[1] 范颖[1] 陈文娜[2] 孙宏伟[2] 马贤德[2] 

机构地区：[1]辽宁中医药大学基础医学院方剂学科,沈阳110847 [2]辽宁中医药大学教学实验中心,沈阳110847

出　　处：《中国实验方剂学杂志》2013年第12期193-196,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金(81001493);教育部博士点新教师基金(20102133120001);辽宁省教育厅基金(L2011162)

摘　　要：目的:观察补肾、健脾和补肾健脾3方对模拟失重大鼠钙、磷代谢的作用,比较其促进骨矿化能力的异同。方法:大鼠随机分为正常对照、悬吊、补肾、健脾和补肾健脾共5组。后4组大鼠头低位-30度尾部悬吊连续21 d模拟失重,补肾、健脾和补肾健脾组大鼠从实验第1天开始依次按2.4,3.2,5.7 g.kg-1.d-1给予补肾方、健脾方和补肾健脾方灌胃,其余各组大鼠灌服等容积的生理盐水。实验第22天取材,双能X射线骨密度仪测左侧胫骨骨密度,全自动生化分析仪检测血和尿中钙磷含量,EDTA法检测粪钙和饲料钙含量。结果:较之正常对照组,悬吊组大鼠左侧胫骨骨密度显著降低(P<0.01),血钙和血磷含量、钙的表观吸收率明显降低(P<0.01),尿钙、尿磷、粪钙含量明显增高(P<0.01);较之悬吊组,补肾健脾组大鼠左侧胫骨骨密度、血钙和血磷含量、摄入钙量、钙的表观吸收率明显升高(P<0.01),尿钙、尿磷、粪钙含量明显降低(P<0.01),补肾组和健脾组大鼠左侧胫骨骨密度、血钙升高(P<0.05),钙的表观吸收率均明显升高(P<0.01),补肾组大鼠血磷含量明显升高(P<0.01),尿钙、尿磷含量显著降低(P<0.01),粪钙含量降低(P<0.05),健脾组大鼠血磷和摄入钙量增加(P<0.05),尿钙、尿磷和粪钙含量降低(P<0.05,P<0.01);较之补肾健脾组,补肾组和健脾组大鼠左侧胫骨骨密度、血钙含量降低(P<0.05),补肾组大鼠钙的表观吸收率明显降低(P<0.01)。结论:补肾、健脾和补肾健脾3方均能改善模拟失重引起的钙磷代谢异常,健脾方在促进钙吸收和维持血钙处于正常水平方面对补肾方具有协同增效作用。Objective： To observe the action of tonifying kidney prescription,invigorating spleen prescription and tonifying kidney and invigorating spleen prescription on the metabolism of calcium and phosphorus in tail suspension model rats.Method： Rats were assigned to five groups randomly： control（C）,tail suspended（S）,tonifying kidney（TK）,invigorating spleen（IS） and tonifying kidney and invigorating spleen（TKIS） groups.Rats in S,TK,IS and TKIS groups were suspended by head down tilt-30° for 21 days.Rats in TK,IS and TKIS groups were orally administrated with three decoctions and other groups with equivalent saline from the first day.Bone mineral density of left tibia was measured respectively using dual energy X ray absorptiometry in rats on day 22.Meanwhile calcium and phosphorus in serum and urine in rats of these five groups were measured by automatic biochemical analyzer.Calcium content in fecal and feed were detected by EDTA.Result： Compared with the C group,bone mineral density,serum calcium and phosphorus and apparent absorption rate of calcium are decreased,urine calcium and phosphorus and fecal calcium were increased obviously in S group.Compared with the S group,the bone mineral density,serum calcium and phosphorus,calcium intake and apparent absorption rate of calcium were raised but urine calcium and phosphorus and fecal calcium are reduced in TKIS group.Bone mineral density,serum calcium and phosphorus and apparent absorption rate of calcium were higher in TK and IS groups than the values in S group.Urine calcium and phosphorus and fecal calcium were lower in TK and IS groups than the values in S group.Calcium intake in IS group is increased above S group.Compared with the TKIS group,bone mineral density and serum calcium were decreased in TK and IS groups and apparent absorption rate of calcium was reduced in TK group.Conclusion： Three prescriptions including tonifying kidney prescription,invigorating spleen prescription and tonifying kidney and invigorating spleen prescriptio

关 键 词：补肾健脾 模拟失重 尾部悬吊 钙代谢 磷代谢 

分 类 号：R285.5[医药卫生—中药学]