农杆菌介导法将抗草甘膦基因转入玉米自交系  

Agrobacterium Tumefaciens Mediated Transformation of Glyphosate Resistant Gene into Maize Inbred Lines

作　　者：王宏伟[1] 吕颖颖[1] 梁业红[2] 史振声[1] 李博[1] 葛云侠[1] 张世煌[2] 

机构地区：[1]沈阳农业大学特种玉米研究所,沈阳110161 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《玉米科学》2013年第3期19-23,共5页Journal of Maize Sciences

基　　金：辽宁省"十二五"农业攻关计划(2011201018)

摘　　要：利用农杆菌介导法将抗草甘膦基因(ssu-G2-aroA)转入优良玉米自交系R18-599和齐319,侵染后的愈伤组织转至潮霉素浓度为10 mg/L的筛选培养基上,继代筛选3轮,每轮3周;得到的抗性愈伤组织在含有潮霉素浓度为3 mg/L的分化培养基上进行分化,R18-599获得30株再生植株,齐319获得18株再生植株。经PCR鉴定后共得到7株转化抗性植株(4株R18-599、3株齐319)。经PCR-Southern及RT-PCR检测结果表明,ssu-G2-aroA基因已稳定整合到了玉米基因组中,并在RNA水平上得到表达。The ssu-G2-aroA gene was transformed into maize inbred lines R18-599 and Qi319 by using Agrobacterium-mediated method. The transformed calli was transferred to the selecting medium with 10 mg/L hygromycin for 9 weeks. Thirty plants of R18-599 and 18 plants of Qi319 were obtained from resistant calli which had been cultured on differentiation medium with 3 mg/L hygromycin. Seven transformants（4 plants R18-599, 3 plants Qi319） were obtained by analyses of PCR, and analyses of PCR-southern and RT-PCR on transformants showed that foreign ssu-G2-aroA gene is integrated into maize genome and is over expressed. The obtained transgenic plants bar boring ssu-G2-aroA.

关 键 词：玉米自交系 农杆菌介导 ssu-G2-aroA基因 

分 类 号：S513.035.3[农业科学—作物学]