香鱼RBP基因的克隆、组织表达特征及其与盐度应激相关的表达分析  

Ayu RBP:molecular cloning,tissue distribution, and its expression in response to salt stress

作　　者：陈强[1] 赵桐[1] 史雨红[1] 陈炯[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《生物学杂志》2013年第3期1-4,23,共5页Journal of Biology

基　　金：国家自然科学基金项目(31201970);浙江省大学生新苗人才计划项目(2012R405042)

摘　　要：血浆视黄醇结合蛋白(Retinol binding protein,RBP)是体内将视黄醇从肝脏转运至靶组织的特异载体蛋白。最近研究发现在盐度升高时肾脏RBP蛋白表达量下降。为了进一步研究香鱼RBP基因mRNA和蛋白表达与盐度应激相关性,从香鱼、肝脏cDNA文库中获得RBP基因cDNA序列。香鱼RBP基因mRNA在肝脏中表达量最高,肾、肠、脑和鳃中表达次之。实时荧光定量RT-PCR结果显示,盐度升高时,RBP基因mRNA表达在不同组织中呈不同下降趋势,其中渗透压调节相关组织鳃、肾中,表达量下降最显著。Western blot实验证实,盐度升高时,香鱼血清RBP蛋白表达量也显著下降。揭示了RBP可能在香鱼盐度适应中有重要作用。Plasma retinol binding protein （RBP） is the specific carrier protein transporting retinol from liver to the target tissues in ver- tebrate plasma. In previous study, RBP in kidney was differently expressed during acclimation from freshwater to brachish water con- firmed by two-dimensional gel electrophoresis. In this study, RBP cDNA was cloned and sequenced for the study of correlation between salt stress and ayu retinol binding protein. Ayu RBP transcripts were determined to be significantly abundant in the liver, and low in the kidney, intestine, brain and gill. RT-qPCR results revealed that ayu RBP mRNA especially in kidney and gill which play vital roles in fish osmoregulation was down-regulated to varying degrees in response to salinity increasing. Western blot analysis showed that the plasma RBP decrased as salinity increased.

关 键 词：视黄醇结合蛋白 克隆 组织差异表达 盐度 香鱼 

分 类 号：Q78[生物学—分子生物学]