石斑鱼属鱼类线粒体基因组序列特征及系统发育信息评估  

Mitochondrial genome characteristics and phylogenetic information of genus Epinephelus(Perciformes:Serranidae) fishes

作　　者：黄小林[1] 吕国敏[1] 刘付永忠[2] 李涛[1] 蔡云川[2] 马志洲[2] 黄忠[1] 

机构地区：[1]中国水产科学研究院南海水产研究所农业部南海渔业资源环境重点野外科学观测试验站.广州510300 [2]广东省水产技术推广总站,广州510222

出　　处：《生物学杂志》2013年第3期9-13,共5页Journal of Biology

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2012TS30,2012TS31);中国水产科学研究院基本科研业务费专项项目(2012A0703);广东省海洋渔业科技推广专项项目(B201101B04)

摘　　要：通过生物信息学方法,比较分析了已登录到GenBank中的6条石斑鱼和1条豹纹鳃棘鲈的线粒体基因组序列,初步揭示了石斑鱼属线粒体基因组的基本特征、蛋白质编码基因、差异位点、选择压力及系统发育等信息。结果表明:1)与大多数脊椎动物一样,石斑鱼属鱼类线粒体基因组均编码37个基因,基因排列顺序与其它硬骨鱼类一致,其中,基因差异位点比例最大为ND6(33.9%),16SrRNA的差异位点比例最小(13.5%);2)6条石斑鱼线粒体基因组中,所有13个蛋白质编码基因的Tajima’s D检测均为不显著的负值,且Ka/Ks均远小于1,都显示出较强的净化选择作用;3)结合分子系统树的置信度和与序列的信息量,对石斑鱼线粒体13个蛋白质编码基因在属内种间的系统进化分析能力进行了评估,结果表明Cytb和ND2基因是理想的分子标记。The structure and organization of mitochondrial genomes of six species of Epinephelus and one Plectropomus leopardus sam- ples were analyzed using bioinformatics method. The basic characteristics including protein coding genes, different loci, selection pres- sure and phylogenetic information were studied in detail. The results showed that mitochondrial genome composition and gene order was consistent with other teleost fishes. There are 37 standard genes; among all the genes, the ND6 had the largest （33.9%） and 16SrRNA had the smallest （13.5%） genetic differences proportion. 13 protein coding genes were mainly acted by purifying selection as detected by Tajima＇ s D test, and all of them were influenced by purifying selection （ Ka/Ks 〈 1 ）. Combined with topological struc- ture and bootstrap value sum of phylogenetic tree. Results showed Cytb and ND2 genes as suitable molecular markers for phylogenetic study of Epinephelus fishes.

关 键 词：石斑鱼属 线粒体基因组 系统发育信息 分子标记 

分 类 号：Q951.3[生物学—动物学]